Effects of Bovine Amelogenin and Enamelin on in vitro Mineralization in Gel

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概要

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• Amelogenins and enamelins are the major extracellular-matrix proteins in developing mammalian enamel. The effects ofthese proteins on mineralization were examined using an in vitro mineralization system in gel. Two proteins, 28kDa amelogenin and 70kDa enamelin, were purified from bovine enamel. Gels containing these proteins were used as diffusion media for calcium and phosphate ions. Calcium phosphate was precipitated in the gels, and was identified as octacalcium phosphate by X-ray and electron diffraction. The precipitation band was sharp in the gel containing the enamelin, although it was broad in the gel containing the amelogenin. Electron microscopic observation showed that crystals precipitating in the former gels were 2-5 times larger than crystals in the later gels. Hydroxyapatite chromatography of these two protcins indicated that the enemelin had a higher affinity to the mineral than the amelogenin. Amelogenin may be responsible for the diffuse nucleation of the crystal, and enamelin may facilitate the localized nucleation and regulate crystal growth.

• 硬組織再生生物学会の論文

著者

• Nagai Noriyuki

  Department of Oral Pathology and Medicine, Graduate School of Medicine, Dentistry & Pharmaceutical S

• SASAKI Satoshi

  Department of Social and Preventive Epidemiology, School of Public Health, The University of Tokyo

• Akao Masaru

  Center Of Oral Health Science International Dental Hygienist College In Okayama Motoyama Gakuen

• Akao Masaru

  Division Of Inorganic Material Engineering Tokyo Medical And Dental University

• Takagi Tohru

  Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A

• Takagi Tohru

  Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S

• Kikuchi Masanori

  Biomaterial Center, National Institute for Materials Science

• Sasaki Satoshi

  Department Of Internal Medicine And Molecular Science Nagoya City University Graduate School Of Medi

• FUJISAWA Ryuichi

  Department of Microbiology, Kinki University School of Medicine

• Nagai Noriyuki

  Department Of Oral Pathology And Medicine Dental School Okayama University

• Akao Masaru

  Institute Of Biomaterials And Bioengineering Tokyo Medical And Dental University

• Fujisawa Ryuichi

  Department Of Biochemistry School Of Dentistry Hokkaido University

• Kikuchi Masanori

  Biomaterials Center National Institute For Materials Science

• KIKUCHI Masanori

  National Institute for Materials Science, Advanced Materials Laboratory, Bioceramics Research Group

• Kikuchi M

  Biomaterials Center National Institute For Materials Science

• Takagi Tohru

  Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S

• Takagi Tohru

  Department Of Biochemistry Faculty Of Dentistry Tokyo Medical And Dental University

• Kikuchi M

  Biomaterial Center National Institute For Materials Science

• Takagi Tohru

  Department Of Oral Pathology And Medicine Okayama University Graduate School Of Medicine Dentistry A

• Kikuchi Masanori

  Crest Jst:biomaterial Center Nims

• Takagi Tohru

  Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni

• Takagi Tohru

  Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S

• Kikuchi Masanori

  Department Of Mineral Resources Engineering School Of Science And Engineering Waseda University

• Takagi Tohru

  Department Of Virology Graduate School Of Medicine Dentistry And Pharmaceutical Sciences Okayama Uni

• Takagi Tohru

  Department Of Oral Pathology And Medicine Graduate School Of Medicine Dentistry And Pharmaceutical S

• Sasaki Satoshi

  Department Of Biochemistry School Of Dentistry Tokyo Medical And Dental University

• Sasaki Satoshi

  Department Of Applied Physics Faculty Of Engineering Nagoya University

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