Laser perforated collagen membrane : pore size-dependent bone induction as a new BMP carrier

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概要

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• Bone morphogenetic protein(BMP)requires a certain carrier to induce bone when implanted into animal tissues such as muscle, skin and periodontal tissues. More than ten different BMP-carriers have been developed to meet different clinical needs. To develop a membranous carrier that is biodegradable, we newly prepared laser perforated collagen membranes(LPM)(35 mm in thickness, non-cross-linked pepsin-treated collagen)with different pore sizes ranging from 0.1 to 1.0 mm in diameter. Geometrical similarity was used to keep a constant ratio of the perforated to the nonperforated areas of the membrane. We compared their efficaies for bone formation in BMP-induced ectopic osteogenesis. The membranes were impregnated with rhBMP-2, implanted subcutaneously into rats and analyzed after being extracted at 1-4 weeks. It was found that all of the LPM showed higher bone-inducing efficacy than the non-perforated collagen membrane as shown by□@histological observations, calcium contents and the alkaline phosphatase level. □@LPM with pore sizes larger than 0.5 mm showed higher amounts of bone than the non-perforated membranes, with the 3.3 times the calcium content(at 3 weeks). These results suggested that the retention of the cytokine and the anchorage of the recruited mesenchymal cells on LPM, particularly with pore sizes larger than 0.5 mm, were higher than those of non-perforated membranes. The LPM is applicable for tissue engineering in the dental field.

• 硬組織再生生物学会の論文

著者

• KIKUCHI Masahiro

  Department of Pathology, School of Medicine, Fukuoka University

• KUBOKI Yoshinori

  Department of Oral Biochemistry, School of Dentistry, Hokkaido University

• Takita Hiroko

  Department Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Takita H

  Central Research Institute

• Takita Hiroko

  Department Of Biochemistry Hokkaido University Dental School

• Nakayama Yasuhide

  Department of Bioengineering, National Cardiovascular Center Research Institute

• Kuboki Y

  Hokkaido University

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University School Of Dentistry

• Kuboki Yoshinori

  Emeritus Professor Hokkaido University

• Kikuchi Masahiro

  Laboratory Of Oral Biochemistry Department Of Oral Health Sciences Graduate School Of Dentistry Hokk

• MATSUDA Takehisa

  Department of Biomedical Engineering, Graduate School of Medicine, Kyushu University

• Matsuda Takehisa

  Dept Bio-engineeting National Cardiovascular Center Research Institute:dept Bio-engineering Kyusyu U

• Matsuda Takehisa

  Department Of Bioengineering National Cardiovascular Center Research Institute

• Kuboki Yoshinori

  Dept. Of Oral Health Science Graduate School Of Dental Medicine Hokkaido University

• Kuboki Yoshinori

  Dept Biochem Sch Dentistry Hokkaido University

• Takita Hiroko

  Dept Biochem Sch Dentistry Hokkaido University

• Kikuchi Masahiro

  Department Of Anatomic And Diagnostic Pathology Dokkyo University School Of Medicine

• Nakayama Yasuhide

  Department Of Bioengineering Advanced Biomedical Engineering Center National Cardiovascular Center R

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University Dental School

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