Porphyromonas gingivalis Protease Degrades Cell Adhesion Molecules of Human Gingival Fibroblasts
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概要
- 論文の詳細を見る
Porphyromonas gingivalis (P. gingivalis), one of the most probable pathogens of periodontal disease, produces several proteases, some of which are generally considered to be pathogenic although details of the mechanism of pathogenesis are still unknown. We analyzed the effect of the purified protease of P. gingivalis on a group of cell adhesion molecules including intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), very late antigen-4 (VLA-4), and fibronectin in human gingival fibroblasts (HGF). Confluent monolayer cultures of HGF were incubated for 24h with P. gingivalis protease. The amounts of ICAM-1, VCAM-1, VIA-4 and fibronectin were analyzed before and after the incubation by flow cytometric analysis of the HGF bound to FITC-conjugated monoclonal antibody. All the cell adhesion molecules examined became immunologically undetectable after 24 h incubation with P. gingivalis protease. These results suggest that degradation of the cell adhesion molecules by the P. gingivalis protease is one of the earliest invasive attacks in periodontal diseases caused by P. gingivalis.
- 硬組織再生生物学会の論文
著者
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Wang Pao-Li
Department of Pharmacology, Matsumoto Dental University
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Wang Pao-li
大阪歯科大学 薬理
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TANI Hiroshi
Department of Mechanical Engineering, Kansai University, High Technology Research Center
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SATO Katsuaki
Department of Applied Physics, Tokyo University of Agriculture and Technology
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Wang P‐l
Osaka Dental Univ. Hirakata Jpn
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KUBOKI Yoshinori
Department of Oral Biochemistry, School of Dentistry, Hokkaido University
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OHURA Kiyoshi
Department of Pharmacology, Osaka Dental University
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Tani Hiroshi
Department Of Preventive Dentistry School Of Dentistry Hokkaido University
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OIDO Mari
Department of Periodontology, Health Sciences University of Hokkaido
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KOWASHI Yuske
Departnent of Periodontology and Endodontology, Health Sciences University of Hokkaido
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Sato Katsuaki
Department Of Cell Processing Institute Of Medical Science University Of Tokyo
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Kuboki Yoshinori
Department Of Biochemistry Hokkaido University School Of Dentistry
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Tani Hiroshi
Depart. Of Mechanical Engineering High Technology Research Center Kansai University
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Oido Mari
Department Of Periodontology And Endodontology School Of Dentistry Health Sciences University Of Hok
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Ohura Kiyoshi
Department Of Pharmacology Osaka Dental University
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Kowashi Yuske
Department Of Periodontology And Endodontology School Of Dentistry Health Sciences University Of Hok
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Wang Pao-li
Department Of Dental Education And Development Osaka Dental University
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Kowashi Yusuke
Department Of Periodontology And Endodontology School Of Dentistry Health Sciences University Of Hok
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Oido Mari
Department Of Periodontology And Endodontology School Of Dentistry Health Sciences University Of Hok
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Ohura K
Department Of Pharmacology Osaka Dental University
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Kuboki Yoshinori
Department Of Biochemistry Hokkaido University Dental School
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Sato Katsuaki
Department of Applied Physics, Graduate School of Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo 184-8588, Japan
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SATO Katsuaki
Department of Applied Physics, Faculty of Technology, Tokyo University of Agriculture and Technology
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