Purification of a Noncollagenous High Molecular Weight Protein from Bovine Articular Cartilage

スポンサーリンク

概要

• 論文の詳細を見る

• A noncollagenous high molecular weight protein (HMW) was isolated from bovine articular cartilage by CsCl density gradient centrifugation followed by DEAE-cellulose chromatography and gel filtration chromatography. The molecular weight was estimated to be 320 kDa and the reduced HMW had a molecular weight of 105 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The amino acid composition of HMW showed a high content of aspartic acid, but no hydroxylysine and hydroxyproline were detected, indicating that the protein was not a collagen. Affinity purified rabbit antibody against HMW reacted specifically with HMW, but there was no immunoreaction with cartilage-specific proteoglycan, type II collagen and type I collagen. HMW showed dose-dependent attachment to types I, II, III and V collagens. However, HMW did not bind to fibronectin, decorin and heparin. Furthermore, the binding of HMW to heat-denatured collagens was lower than native collagens. In these observation, HMW may be recognized the triple helix structure of collagen for the interaction.

• 公益社団法人日本薬学会の論文
• 1996-01-15

著者

• ONODERA Satoshi

  Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University

• TASHIRO Shinichi

  Department of Clinical and Biomedical Science, Showa Pharmeceutical University

• Onodera Satoshi

  Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University

• KUBOKI Yoshinori

  Department of Oral Biochemistry, School of Dentistry, Hokkaido University

• Kuboki Yoshinori

  Department Of Biochemistry School Of Dentistry Hokkaido University

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University School Of Dentistry

• Tashiro Shinichi

  Department Of Clinical And Biomedical Sciences Showa College Of Pharmaceutical Sciences

• Kuboki Yoshinori

  Department Of Biochemistry Hokkaido University Dental School

関連論文

• Silibinin Induced Autophagic and Apoptotic Cell Death in HT1080 Cells Through a Reactive Oxygen Species Pathway
• The Protective Effect of Silibinin Against Mitomycin C-Induced Intrinsic Apoptosis in Human Melanoma A375-S2 Cells
• Bcl-2 Family Proteins Were Involved in Pseudolaric Acid B-Induced Autophagy in Murine Fibrosarcoma L929 Cells
• A Comparative Study of the Usefulness of Toki-shakuyaku-san and An Oral Iron Preparation in the Treatment of Hypochromic Anemia in Cases of Uterine Myoma
• Caspase Inhibition Augmented Oridonin-Induced Cell Death in Murine Fibrosarcoma L929 by Enhancing Reactive Oxygen Species Generation
• Reactive Oxygen Species Mediate Oridonin-Induced HepG2 Apoptosis Through p53, MAPK, and Mitochondrial Signaling Pathways
• Inhibition of Insulin-like Growth Factor 1 Receptor Signaling Enhanced Silibinin-Induced Activation of Death Receptor and Mitochondrial Apoptotic Pathways in Human Breast Cancer MCF-7 Cells
• Oridonin Induced Autophagy in Human Cervical Carcinoma HeLa Cells Through Ras, JNK, and P38 Regulation
• Inactivation of Ras and Changes of Mitochondrial Membrane Potential Contribute to Oridonin-Induced Autophagy in A431 Cells
• Autophagy Preceded Apoptosis in Oridonin-Treated Human Breast Cancer MCF-7 Cells(Molecular and Cell Biology)
• Oridonin Inhibited the Tyrosine Kinase Activity and Induced Apoptosis in Human Epidermoid Carcinoma A431 Cells(Molecular and Cell Biology)
• Oridonin-Induced A431 Cell Apoptosis Partially Through Blockage of the Ras/Raf/ERK Signal Pathway
• Silibinin Protects Against Isoproterenol-Induced Rat Cardiac Myocyte Injury Through Mitochondrial Pathway After Up-regulation of SIRT1
• Fibroblast Growth Factor-2 Suppresses Oridonin-Induced L929 Apoptosis Through Extracellular Signal-Regulated Kinase-Dependent and Phosphatidylinositol 3-Kinase-Independent Pathway
• Silibinin Protects Rat Cardiac Myocyte from Isoproterenol-Induced DNA Damage Independent on Regulation of Cell Cycle(Pharmacology)
• Fas/FasL Signaling Allows Extracelluar-Signal Regulated Kinase to Regulate Cytochrome c Release in Oridonin-Induced Apoptotic U937 Cells(Pharmacology)
• Augmentation of Oridonin-Induced Apoptosis Observed With Reduced Autophagy
• Silibinin Prevents UV-Induced HaCaT Cell Apoptosis Partly through Inhibition of Caspase-8 Pathway(Pharmacology)
• Bcl-2 Up-Regulation and P-p53 Down-Regulation Account for the Low Sensitivity of Murine L929 Fibrosarcoma Cells to Oridonin-Induced Apoptosis(Pharmacology)
• Activation of Phosphoinositide 3-Kinase, Protein Kinase C, and Extracellular Signal-Regulated Kinase Is Required for Oridonin-Enhanced Phagocytosis of Apoptotic Bodies in Human Macrophage-Like U937 Cells
• Evodiamine Induced Human Melanoma A375-S2 Cell Death Partially through Interleukin 1 Mediated Pathway(Pharmacology)
• (+)-Catechin, an Ingredient of Green Tea, Protects Murine Microglia From Oxidative Stress-Induced DNA Damage and Cell Cycle Arrest
• A Comparative Study of Laboratory Parameters and Symptoms Effected by Toki-shakuyaku-san and an Iron Preparation in Rats with Iron-Deficiency Anemia(Biopharmacy)
• Effects of Liver-Supplemented Food on the Development of Embryos in Mice
• Silibinin Activated p53 and Induced Autophagic Death in Human Fibrosarcoma HT1080 Cells via Reactive Oxygen Species-p38 and c-Jun N-Terminal Kinase Pathways
• O12-009 後発品使用促進の現状と問題点 : 後発医薬品は薬剤師に受け入れられているのか?(一般演題 口頭発表,糖尿病/使用状況調査・意識調査,医療薬学の創る未来 科学と臨床の融合)
• 軟骨細胞が合成するCOMP(Cartilage Oligomeric Matrix Protein)の性状解析
• Effect of Blended Ionomers on the Strain Hardening of Polyester-Type Elastomer/Ionomer Blends
• The relationship between 1-deoxynojirimycin content and α-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) of Kyoto, Japan
• Dynamic Responses to Acute Heat Stress between 34 ℃ and 38.5 ℃, and Characteristics of Heat Stress Response in Mice
• Increases in Plasma Dihydroxyphenylacetic Acid Levels in Decapitated Mice after Exposure to Various Stresses
• Combination of Recombinant Human BMP-2 and Atelocollagen Implant with Cortical Perforations for Bone Augmentation
• Histopathological Studies of Heterotopic Bone Formations Induced by BMP Carrier Composites
• Simple and Rapid Purification of Histatins Using Hydroxyapatite Chromatography in Denaturalized Conditions
• Measurement of Histatins in the Saliva of Healthy Humans
• Destruction of LPS Receptor by Porphyromonas gingivalis Protease is Prevented by Histatin 5, a Specific Protease Inhibitor
• Porphyromonas gingivalis Protease Degrades Cell Adhesion Molecules of Human Gingival Fibroblasts
• mRNA Expression of MT1-MMP, MMP-9,Cathepsin K, and TRAP in Highly Enriched Osteoclasts Cultured on Several Matrix Proteins and Ivory Surfaces
• Time-Dependent Expression of Bone Sialoprotein Fragments in Osteogenesis Induced by Bone Morphogenetic Protein^1
• Purification of a Noncollagenous High Molecular Weight Protein from Bovine Articular Cartilage
• Geometry of Artificial ECM : Sizes of Pores Controlling Phenotype Expression in BMP-Induced Osteogenesis and Chondrogenesis
• Inhibition of BMP-Induced Ectopic Bone Formation by an Antiangiogenic Agent (Epigallocatechin 3-Gallate)
• Laser perforated collagen membrane : pore size-dependent bone induction as a new BMP carrier
• 8. Regeneratlon of cementum and periodontal ligament by BMP-applied tissue engineering.
• Expression of COMP and proteoglycan in the cartilage differentiation process of chondrocyte stem cell ATDC5 cell
• Dracorhodin Perchlorate Induces A375-S2 Cell Apoptosis via Accumulation of p53 and Activation of Caspases(Pharmacology)
• Cytotoxic Effects of Mansonone E and F Isolated from Ulmus pumila(Pharmacology)
• Dracorhodin Perchlorate Induces Apoptosis via Activation of Caspases and Generation of Reactive Oxygen Species
• Intracellular Regulation of Evodiamine-Induced A375-S2 Cell Death(Pharmacology)
• Roles of SIRT1 and Phosphoinositide 3-OH Kinase/Protein Kinase C Pathways in Evodiamine-Induced Human Melanoma A375-S2 Cell Death
• Roles of SIRT1 and Phosphoinositide 3-OH Kinase/Protein Kinase C Pathways in Evodiamine-Induced Human Melanoma A375-S2 Cell Death
• Evaluation of the Susceptibility of Dentin Phosphoproteins to Proteases and Heat Treatment
• Oridonin Enhances Phagocytosis of UV-Irradiated Apoptotic U937 Cells(Pharmacology)
• Cytochrome c Release From Oridonin-Treated Apoptotic A375-S2 Cells Is Dependent on p53 and Extracellular Signal-Regulated Kinase Activation
• Oridonin Induces a Caspase-Independent But Mitochondria- and MAPK-Dependent Cell Death in the Murine Fibrosarcoma Cell Line L929(Pharmacology)
• Silymarin Prevents UV Irradiation-Induced A375-S2 Cell Apoptosis(Pharmacology)
• p53-Mediated Cell Cycle Arrest and Apoptosis Induced by Shikonin via a Caspase-9-Dependent Mechanism in Human Malignant Melanoma A375-S2 Cells
• 1. The importance of geometrical properties of carrier in BMP-in duced bone formation(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)
• Pleiotrophin Regulates Bone Morphogenetic Protein (BMP)-Induced Ectopic Osteogenesis
• -0938-ANGIOTENSIN II STIMULATES PROTEIN SYNTHESIS IN CARDIAC FIBROBLASTS OF CARDIOMYOPATHIC HAMSTERS : THE 54th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -0512-ANGIOTENSIN II POTENTIATES EXTRACELLULAR MATRIX PRODUCTION IN THE MYOCARDIUM OF SPONTANEOUSLY HYPERTENSIVE RATS
• -0029-ANALYSIS OF AMOUNT, TYPES AND REDUCIBLE CROSSLINKS OF CARDIOMYOPATHIC (BIO 14.6) AND NORMAL(FIB) HAMSTERS
• Geometric Effect of Matrix upon Cell Differentiation : BMP-Induced Osteogenesis Using a New Bioglass with a Feasible Structure^1
• 15. Bone formation induced by the implantation of bone marrow cell collagen matrix complexes in vivo.
• Characteristic Changes of Reducible Collagen Cross-links to the Hard Tissue Pattern in Turkey Tendon upon Calcification : Nature of Calcifiable Collagen
• Effects of mechanical stress on the eryression of type XII collagen mRNA in human periodontal ligament cells
• Acidic Homopolypeptides as Models for Hydroxyapatite-Binding Sites of Non-collagenous Proteins in Bone and Tooth
• Type I Collagen Matrix and β-glycerophosphate Facilitate Mineralized Tissue Formation by Rat Dental Pulp Cells
• Anti-Bone Sialoprotein (BSP) Monoclonal Antibody Suppressed the Expression of Osteoblastic Phenotypes of Bone Marrow Stromal Cells Cultured on Type I Collagen Matrix
• Sensitive Staining of Acidic Dentin Proteins with Electrophoresis
• A Synthetic Peptide That Mediate Attachment of Osteoblastic Cells to Mineral Crystal
• Osteoblast-Related Gene Expression of Bone Marrow Cells during the Osteoblastic Differentiation Induced by Type I Collagen
• Effect of BMP on the differentiation of bone marrow stromal cells into osteoblasts in a collagen-gel culture
• 9. Discovery and significance of the calcium-induced precipitation of enamel proteins.
• Effect of Histatin 5, a Specific Protease Inhibitor, on Cytotoxic Activity of Porphyromonas gingivalis Protease in Cultured Human Gingival Fibroblasts
• Effects of LPS and Protease from Porphyromonas gingivalis on Cultured Human Gingival Fibroblasts
• Mechanism of Incorporation of Histones into Bone Matrix
• Changes of Noncollagenous Extracellular Matrix in Turkey Leg Tendon During Calcification
• Immunolocalization of chondroadherin in the human femur
• Biochemical analysis of collagen in adhesive tissues formed after digital flexor tendon injuries
• Pore Size of Porous Hydroxyapatite as the Cell-Substratum Controls BMP-Induced Osteogenesis^1
• Changes of the Rat Dentin Matrix Proteins Affected by Long-term Administration of Hydroxyethylidene-1, 1-bisphosphonate (HEBP)
• 8. An in vitro mineralization system in gel for micro amount of proteins
• Silibinin Induced Autophagic and Apoptotic Cell Death in HT1080 Cells Through a Reactive Oxygen Species Pathway
• BMP-Induced Osteogenesis with Two Geometrically Different Biodegradable Carbonate Apatites
• Isolation and Immunochemical Characterization of Heparan Sulfate Rich Proteoglycan (HSPG) Present in the Basement Membrane of Human Placenta
• Effect of External Fluctuation Frequency Introduced in the Mean-Flow on the Characteristic Frequency of Separated Flow
• 9. The passaged bone marrow cells lose the activity of differentiarion to osteoblasts(8th Annual Meeting of the Society of Hard Tissue Biology July 24, 1999 in Asahikawa)
• TNFα-Induced Necroptosis and Autophagy via Supression of the p38–NF-κB Survival Pathway in L929 Cells
• Subnanogram detection of highly phosphorylated proteins immobilized on membranes
• TNFα-Induced Necroptosis and Autophagy via Supression of the p38-NF-κB Survival Pathway in L929 Cells
• Activation of ERK-p53 and ERK-Mediated Phosphorylation of Bcl-2 Are Involved in Autophagic Cell Death Induced by the c-Met Inhibitor SU11274 in Human Lung Cancer A549 Cells
• Interruption of Hepatocyte Growth Factor Signaling Augmented Oridonin-Induced Death in Human Non-small Cell Lung Cancer A549 Cells via c-Met-Nuclear Factor-κB-Cyclooxygenase-2 and c-Met-Bcl-2-Caspase-3 Pathways
• Activated O_2^ and H_2O_2 Mediated Cell Survival in SU11274-Treated Non-Small-Cell Lung Cancer A549 Cells via c-Met-PI3K-Akt and c-Met-Grb2/SOS-Ras-p38 Pathways
• Activated O2•− and H2O2 Mediated Cell Survival in SU11274-Treated Non-Small-Cell Lung Cancer A549 Cells via c-Met–PI3K–Akt and c-Met–Grb2/SOS–Ras–p38 Pathways
• Dracorhodin Perchlorate Induces Apoptosis via Activation of Caspases and Generation of Reactive Oxygen Species
• Hydroxyl Radical (·OH) Played a Pivotal Role in Oridonin-Induced Apoptosis and Autophagy in Human Epidermoid Carcinoma A431 Cells
• Pseudolaric Acid B–Induced Autophagy Contributes to Senescence via Enhancement of ROS Generation and Mitochondrial Dysfunction in Murine Fibrosarcoma L929 Cells
• Pseudolaric Acid B-Induced Autophagy Contributes to Senescence via Enhancement of ROS Generation and Mitochondrial Dysfunction in Murine Fibrosarcoma L929 Cells

もっと見る 閉じる

スポンサーリンク