Oridonin-Induced A431 Cell Apoptosis Partially Through Blockage of the Ras/Raf/ERK Signal Pathway
スポンサーリンク
概要
- 論文の詳細を見る
We have reported that oridonin, a diterpenoid isolated from the plant Rabdosia rubescens, had apoptosis-inducing activities in many cell lines (e.g., human melanoma A375-S2, human cervical cancer HeLa, human breast adenocarcinoma MCF-7, and murine fibrosarcoma L929). In this study, we further investigated signaling events involved in oridonin-induced apoptosis in human epidermoid carcinoma A431 cells. It was found that the total tyrosine kinase activity was inhibited and the protein expressions of epidermal growth factor receptor (EGFR) and phosphorylated EGFR were decreased in oridonin-induced A431 cell apoptosis. Expression of EGFR downstream effector proteins, Grb2, Ras, Raf-1, and extracellular signal-regulated kinase (ERK), was also downregulated by oridonin. Moreover, the oridonin-induced apoptosis was augmented by the Ras inhibitor manumycin A, Raf-1 inhibitor GW5074, or ERK inhibitor PD98059, suggesting that inactivation of Ras, Raf, or ERK participates in oridonin-induced apoptosis. Taken together, oridonin-induced apoptosis in A431 cells might be through blocking EGFR and its downstream Ras/Raf/ERK signal pathway.
- 社団法人 日本薬理学会の論文
- 2007-01-20
著者
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Tashiro Shin-ichi
Dep. Of Clinical And Biomedical Sciences Showa Pharmaceutical Univ. Jpn
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TASHIRO Shin-ichi
Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University
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ONODERA Satoshi
Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University
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IKEJIMA Takashi
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical Unive
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Wu Li-jun
Department Of Phytochemistry Shenyang Pharmaceutical University
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Wu L
Department Of Phytochemistry Shenyang Pharmaceutical University
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Onodera S
Dep. Of Clinical And Biomedical Sciences Showa Pharmaceutical Univ. Jpn
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Onodera Satoshi
昭和薬科大学 病態科
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Ikejima Takashi
China-japan Res. Inst. Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical Univ. Chn
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LI Dan
China-Japan Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University
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Tashiro Shin-ichi
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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Onodera Satoshi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Tashiro Shin-ichi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Li Dan
China-japan Research Institute Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical Univer
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Tashiro S
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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Onodera S
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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Ikejima Takashi
China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University
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