Oridonin Induced Autophagy in Human Cervical Carcinoma HeLa Cells Through Ras, JNK, and P38 Regulation
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概要
- 論文の詳細を見る
In this study, we investigated autophagy induced by oridonin in HeLa cells. HeLa cells were exposed to oridonin, and the fluorescent changes, autophagic levels, and protein expressions were evaluated. Oridonin induced autophagy in HeLa cells in vitro in a dose- and time-dependent manner. Oridonin-treated HeLa cells, which had been prelabeled with the autophagosome-specific dye monodansylcadervarine (MDC), recruited more MDC-positive particles and had a significantly higher fluorescent density; and simultaneously, expressions of autophagy-related proteins, MAP-LC3 and Beclin 1, were increased by oridonin. In oridonin-induced Hela cells, pretreatment with 3-methyladenine (3-MA, the specific inhibitor of autophagy) dose-dependently decreased the autophagic ratio accompanied with downregulation of the protein expressions of MAP-LC3 and Beclin 1. Furthermore, when a Ras inhibitor was applied, the autophagic levels were augmented, whereas P38 and JNK inhibitors decreased the autophagic ratio significantly, indicating that this oridonin-induced autophagic process was negatively regulated by Ras, but positively regulated by P38 and JNK MAPKs. Raf-1 and ERK1/2 had no obvious correlation to these signaling pathways.
- 社団法人 日本薬理学会の論文
- 2007-12-20
著者
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Tashiro Shin-ichi
Dep. Of Clinical And Biomedical Sciences Showa Pharmaceutical Univ. Jpn
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TASHIRO Shin-ichi
Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University
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ONODERA Satoshi
Department of Clinical and Biomedical Sciences, Showa Pharmaceutical University
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IKEJIMA Takashi
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical Unive
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Onodera S
Dep. Of Clinical And Biomedical Sciences Showa Pharmaceutical Univ. Jpn
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Onodera Satoshi
昭和薬科大学 病態科
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Ikejima Takashi
China-japan Res. Inst. Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical Univ. Chn
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CUI Qiao
China-Japan Research Institute of Medical Pharmaceutical Sciences, Shenyang Pharmaceutical Universit
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MINAMI Mutsuhiko
Department of Immunology, Yokohama City University School of Medicine
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Tashiro Shin-ichi
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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Onodera Satoshi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Tashiro Shin-ichi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Cui Qiao
China-japan Institute Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical University
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Tashiro S
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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Onodera S
Department Of Clinical And Biomedical Sciences Showa Pharmaceutical University
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MINAMI Matsuhiko
Department of Immunology, Yokohama City University School of Medicine
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Minami Matsuhiko
Department Of Immunology Yokohama City University School Of Medicine
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Minami Mutsuhiko
Department Of Immunology Yokohama City University School Of Medicine
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Ikejima Takashi
China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University
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