Pseudolaric Acid B–Induced Autophagy Contributes to Senescence via Enhancement of ROS Generation and Mitochondrial Dysfunction in Murine Fibrosarcoma L929 Cells

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概要

• 論文の詳細を見る

• Pseudolaric acid B (PAB) is the primary biologically active compound isolated from the root bark of P. kaempferi G<span style="font-variant: small-caps;">ordon</span>. Our previous study demonstrated that PAB induced mitotic catastrophe in L929 cells and indicated that only a small percentage (12%) of the cells undergoing mitotic catastrophe displayed an apoptotic phenotype after PAB treatment for 72 h. In this study, we found that a minority of the cells undergoing mitotic catastrophe ended in apoptosis, and a majority of them entered a period of senescence. Further data confirmed that PAB induced autophagy, reactive oxygen species (ROS) generation, and mitochondrial dysfunction in L929 cells. Subsequently, we found that autophagy inhibitors significantly delayed the senescence process, indicating that autophagy facilitated senescence. Moreover, ROS scavenger significantly decreased the autophagic level and improved mitochondrial function. Additionally, autophagy inhibitors effectively reduced ROS levels and ameliorated mitochondrial function. In conclusion, autophagy promoted senescence via enhancement of ROS generation and mitochondrial dysfunction in PAB-treated L929 cells.

• 公益社団法人 日本薬理学会の論文

著者

• Onodera Satoshi

  Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University

• Xia Mingyu

  China-japan Research Institute Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical Univer

• Qi Min

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University

• Ikejima Takashi

  China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University

• Tashiro Shin-ichi

  Institute for Clinical and Biomedical Sciences, Japan

• Fan Simiao

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

• Xia Mingyu

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

• Ikejima Takashi

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

• Li Zhao

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

• Yao Guodong

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

• Zhou Haiyan

  China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University, China

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