Dracorhodin Perchlorate Induces Apoptosis via Activation of Caspases and Generation of Reactive Oxygen Species
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概要
- 論文の詳細を見る
Dracorhodin perchlorate inhibited proliferation of several tumor cell lines. The drug induced oligonucleosomal fragmentation of DNA in HeLa cells and increased caspase-3, -8, -9 activities followed by the degradation of caspase-3 substrates, inhibitor of caspase-dependent DNase, and poly-(ADP-ribose) polymerase. It also increased caspase-1 activity and a caspase-1 inhibitor, Ac-YVAD-cmk, and a caspase-10 inhibitor z-AEVD-fmk, also reduced dracorhodin-perchlorate-induced HeLa cell death. Dracorhodin perchlorate decreased the expression of anti-apoptotic mitochondrial protein, Bcl-X<sub>L</sub>, but not Bcl-2; and it increased the expression of pro-apoptotic protein, Bax. Dracorhodin perchlorate induced a sustained generation of reactive oxygen species (ROS) in HeLa cells; caspase-1 inhibitor, Ac-YVAD-cmk, and caspase-3 inhibitor, z-DEVD-fmk, attenuated the generation of ROS. Taken together, our results indicate that dracorhodin perchlorate alters the intracellular redox status, changed the balance of Bcl-X<sub>L</sub> and Bax protein expression, and induces apoptosis through caspase pathways in HeLa cells.<br>
著者
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Onodera Satoshi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Tashiro Shin-ichi
Department Of Cinical And Biomedical Sciences Showa Pharmaceutical University
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Xia Mingyu
China-japan Research Institute Of Medical And Pharmaceutical Sciences Shenyang Pharmaceutical Univer
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Wang Minwei
Department Of Pharmacology School Of Life Science And Biopharmaceutics Shenyang Pharmaceutical Unive
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Wang Dong
Department Of Anatomy Histology And Embryology Chinese Academy Of Medical Sciences Peking Union Medi
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Minami Mutsuhiko
Department Of Immunology Yokohama City University School Of Medicine
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Ikejima Takashi
China–Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University
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Xia Mingyu
China-Japan Research Institute of Medical and Pharmaceutical Sciences, Shenyang Pharmaceutical University
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Wang Dong
Department of Pharmacognosy, Shenyang Pharmaceutical University
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