Mechanism of Incorporation of Histones into Bone Matrix
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概要
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Histones were detected in the HCI extract of bone matrix. The mechanism of their incorporation into the matrix was investigated by in vitro binding experiments. We purified from the HCI extract of bovine bone a group of basic proteins, which were identified as histones by Western blotting using antihistone antibody. Histones were incubated with synthetic hydroxyapatite, and were found to be bound to the crystals. Bone non-collagenous proteins were separated by gel electrophoresis and transferred fo a membrane. The membrane was incubated with histones, and the histones bound were detected with antihistone antibody. Histones were specifically bound to proteoglyoans. Histones derived from dead bone cells may be incorporated into bone matrix by their affinity to hydroxyapatite and/or to proteoglycans.
- 硬組織再生生物学会の論文
著者
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Liu An-jun
Sangi Co. Sapporo Laboratory:department Of Biochemistry School Of Dentistry Hokkaido University
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KUBOKI Yoshinori
Department of Oral Biochemistry, School of Dentistry, Hokkaido University
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FUJISAWA Ryuichi
Department of Microbiology, Kinki University School of Medicine
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Kuboki Yoshinori
Department Of Biochemistry Hokkaido University School Of Dentistry
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Fujisawa Ryuichi
Department Of Biochemistry School Of Dentistry Hokkaido University
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Kuboki Yoshinori
Department Of Biochemistry Hokkaido University Dental School
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FUJISAWA Ryuichi
Department of Biochemistry, School of Dentistry, Hokkaido University
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