Preventive Effects of a Kampo Medicine, Shosaikoto, on Inflammatory Responses in LPS-Treated Human Gingival Fibroblasts(Pharmacology)
スポンサーリンク
概要
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In the present study, we investigated the anti-inflammatory effects of a Kampo medicine Shosaikoto (TJ-9) using in vitro periodontal disease model, in which human gingival fibroblasts (HGFs) treated with lipopolysaccharide (LPS) from Porphyromonas gingivalis (PgLPS) produce IL-6, IL-8 and prostaglandin E_2 (PGE_2). Treatment with PgLPS (10 ng/ml), TJ-9 (up to 1 mg/ml) and their combinations for 24 h did not affect the viability of HGFs. Moreover, TJ-9 did not alter LPS-induced IL-6 and IL-8 productions. However, TJ-9 significantly suppressed LPS-induced PGE_2 production in a dose-dependent manner but TJ-9 alone did not affect basal PGE_2 level. Western blotting demonstrated that TJ-9 decreased cyclooxygenase-2 (COX-2) expression in a dose-dependent manner but not phospholipase A_2. Moreover, TJ-9 selectively and dose-dependently inhibited COX-2 activity. These results suggest that TJ-9 decreased PGE_2 production by inhibition of both COX-2 expression and activity and that TJ-9 may be useful to improve gingival inflammation in periodontal disease.
- 2008-06-01
著者
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Ara Toshiaki
Department of Pharmacology, Matsumoto Dental University
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Fujinami Yoshiaki
Department of Pharmacology, Matsumoto Dental University
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Hattori Toshimi
Department of Pharmacology, Matsumoto Dental University
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Imamura Yasuhiro
Department of Pharmacology, Matsumoto Dental University
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Wang Pao-Li
Department of Pharmacology, Matsumoto Dental University
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Wang Pao-li
松本歯科大学 薬理学講座
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Wang Pao-li
Dep. Of Pharmacology Matsumoto Dental Univ.
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Hattori Toshimi
Dep. Of Pharmacology Matsumoto Dental Univ.
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Hattori Toshimi
Department Of Dental Pharmacology Matsumoto Dental University
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Imamura Yasuhiro
Dep. Of Pharmacology Matsumoto Dental Univ.
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MAEDA Yoshihiro
Department of Hard Tissue Research, Graduate School of Oral Medicine, Matsumoto Dental University
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Maeda Yoshihiro
Department Of Hard Tissue Research Graduate School Of Oral Medicine Matsumoto Dental University
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Fujigaki Yoshihisa
Department Of Oral Preventive Dentistry Matsumoto Dental University
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Ara Toshiaki
Department Of Pharmacology Matsumoto Dental University
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Imamura Yasuhiro
Department Of Internal Medicine Kagoshima Kouseiren Hospital
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Ara Toshiaki
Department Of Pharmacology Graduate School Of Oral Medicine Matsumoto Dental University
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Wang Pao-li
Department Of Dental Education And Development Osaka Dental University
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MAEDA Yoshihiro
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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