Interleukin-8 Production by Human Gingival Fibroblasts in Response to Periodontopathic Bacterial Lipopolysaccharides and Prostaglandin E_2 In vitro.
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概要
- 論文の詳細を見る
This study examined the modulating effect of PGE_2 on LPS-induced IL-8 production by human gingival fibroblasts in vitro. The expression of IL-8 mRNA, after stimulation with PGE_2, was determined by RT-PCR. IL-8 production by HGFs was shown to be related to the time of incubation and the dose of LPS. A slight increase in IL-8 levels was observed when cells were exposed to PGE_2 alone compared to controls. When HGFs were pre-stimulated with PGE_2 and then exposed to LPS, IL-8 production was increased 2-3 fold compared to non-treated cells. Herbimycin A completely abolished the secretion of IL-8. These results indicate that PGE_2 can modulate cytokine secretion from HGFs and increase IL-8 production from cells exposed to LPS. IL-8 production induced by LPS was dependent on the tyrosine phosphorylation pathway.
- 硬組織再生生物学会の論文
著者
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Wang Pao-li
Departments Of Pharmacology And Periodontology Matsumoto Dental University
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Yajima Toshihiko
Institute Of Medical Science And Department Of Oral Anatomy Health Sciences University Of Hokkaido
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Ota Norio
Departments Of Pharmacology And Periodontology Matsumoto Dental University
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Ota Norio
Department Of Periodontology Matsumoto Dental University School Of Dentistry
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FUJII Takeo
Institute of Symbiotic Science and Technology, Tokyo University of Agriculture and Technology
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Wang Pao-li
Department Of Dental Education And Development Osaka Dental University
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Carrel-Geinoz Anne
Department of Preventive Dentistry, School of Dental Medicine, University of Geneva
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Usui Shuhei
Departments of Pharmacology and Periodontology, Matsumoto Dental University
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Otogoto Junichi
Departments of Pharmacology and Periodontology, Matsumoto Dental University
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Baehni Pierre
Department of Preventive Dentistry, School of Dental Medicine, University of Geneva
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Usui Shuhei
Departments Of Pharmacology And Periodontology Matsumoto Dental University
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Baehni Pierre
Department Of Preventive Dentistry School Of Dental Medicine University Of Geneva
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Otogoto Junichi
Departments Of Pharmacology And Periodontology Matsumoto Dental University
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Carrel-geinoz Anne
Department Of Preventive Dentistry School Of Dental Medicine University Of Geneva
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Fujii Takeo
Institute Of Medical Science Health Sciences University Of Hokkaido:department Of Pathology And Oper
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