In vitro Osteogenic Differentiation Potential of Dental Pulp Stem Cells
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概要
- 論文の詳細を見る
In the present study, the in vitro osteogenic differentiating potential of rat dental pulp stem cells was examined. The induction was carried out under the same inducing system as used for bone marrow cells. Alkaline phosphatase (ALP) activity and mineralization were measured at 1, 2, 3, and 6 weeks after the induction. The level of ALP activity was very high at 1 week after induction and the level was maintained for 3 weeks, however the level of ALP activity was dramatically decreased (to 50%) after 6 weeks. Although mineralization was detected at low levels after 3 weeks, it became expanded to up to 29 nodules mineralized at 6 weeks after induction, as determined by von Kossa staining. It was shown that in vitro osteogenic differentiation of rat dental pulp stem cells was induced following the increase of the ALP activity at an early stage of the induction.
- 日本再生歯科医学会の論文
- 2004-12-30
著者
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TAKAHASHI TOSHIFUMI
Department of Bioengineering, Tokyo Institute of Technology
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WATANABE Masatoshi
Laboratory for Medical Engineering, Division of Materials Science and Chemical Engineering, Graduate
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DAITO Michiharu
Department of Pediatric Dentistry, Osaka Dental University
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SHINOHARA Mitsuko
Department of Pharmacology, Osaka Dental University
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OHURA Kiyoshi
Department of Pharmacology, Osaka Dental University
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Daito M
Osaka Dental Univ. Osaka Jpn
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Daito Michiharu
Department Of Pediatric Dentistry Osaka Dental University
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TAKEYASU Masaharu
Department of Pediatric Dentistry, Osaka Dental University
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Takahashi Toshifumi
Department Of Pharmacology Osaka Dental University
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Takahashi Toshifumi
Department Of Anesthesia Gunma Cancer Center
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Watanabe Masatoshi
Laboratory For Medical Engineering Division Of Materials Science And Chemical Engineering Graduate S
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Morita Joji
Second Department Of Pathology Mie University School Of Medicine
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Date Masataka
Department Of Pharmacology Osaka Dental University
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Nagata Sachiko
Department Of Pediatric Dentistry Graduate School Of Dentistry Osaka Dental University
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Ohura Kiyoshi
Department Of Pharmacology Osaka Dental University
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Iwamoto Kazuya
Department Of Pharmacology Osaka Dental University
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Iwamoto Kazuya
Department Of Internal Medicine Kobe Red Cross Hospital
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NOZAKI Tadashige
Department of Pharmacology, Osaka Dental University
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HIDAKA Atsushi
Department of Pharmacology, Osaka Dental University
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Hidaka Atsushi
Department Of Pharmacology Osaka Dental University
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Shinohara Mitsuko
Department Of Pharmacology Osaka Dental University
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Takeyasu Masaharu
Department Of Pediatric Dentistry Osaka Dental University
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Daito Miho
Department Of Pediatric Dentistry Osaka Dental University
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Shinohara M
Osaka Dental Univ. Osaka Jpn
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Watanabe Masatoshi
Laboratory For Medical Engineering Division Of Materials Science And Chemical Engineering Graduate S
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Nozaki Tadashige
Department Of Pharmacology Osaka Dental University
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Watanabe Masatoshi
Laboratory For Medical Engineering Division Of Materials And Chemical Engineering Yokohama National University
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