Effect of bio-catalyzer on macrophage function in ODUS/Odu

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Bio-catalyzer (BN) is a product of the yeast fermentation of Carica papaya, Linn, and is a healthy, natural food sold in Japan for human consumption. It is a free radical scavenger, and inhibits bacterial growth and the action of toxohormone-L. Since free radicals are involved in the development of periodontal disease, we investigated the effect of BN on periodontal disease using rats with naturally occurring gingivitis (ODUS/Odu). We treated ODUS/Odu with BN in vivo and in vitro and investigated its effect on chemotaxis, phagocytosis, and the superoxide anion production of peritoneal macrophages. The in vivo experimental group was fed a normal diet with 2% BN in water and the control group was given the same diet with tap water for 10 weeks. We measured the body weight, pocket probing depths, and the effect on macrophage function for all animals. Macrophage chemotaxis was measured by the membrane filter method, and examination of macrophage phagocytosis was performed using fluorescein conjugated Escherichia coli. After two weeks, the pocket probing depth of the BN group was less than that of the controls. Macrophage chemotaxis was significantly lower in the BN-treated group than in the controls at 10 weeks in 1 : 20 zymosan activated serum (ZAS) (p<0.05). Macrophage phagocytosis was significantly lower in the BN group than in the control group at 4 weeks (p<0.01) and 10 weeks (p<0.05). We made various concentrations of BN, and put them in direct contact in vitro with macrophages. We then studied the effects of BN on chemotaxis, phagocytosis and superoxide anion production. Macrophage superoxide anion production of phorbol myristate acetate (PMA) was more suppressed after three hours in the experimental groups than in the controls except at 0.1 ng/mL. At 1 and 10 ng/mL the significance was p<0.05, and at 1 ×10^2, 1 ×10^3 and 1×10^4 ng/mL it was p<0.01. This suggests that BN plays an immunological role in host defense.

Effect of bio-catalyzer on macrophage function in ODUS/Odu

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