Effects of streptozotocin-induced diabetes in neonatal rats with naturally occurring gingivitis

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Rats with non-insulin-dependent diabetes mellitus (NIDDM) induced by neonatal streptozotocin injection are a useful animal model. We administered an intraperitoneal injection at 65 mg/kg of streptozotocin dissolved in 0.05 M citrate buffer at pH 4.5 to neonatal rats with naturally occurring gingivitis rat (ODUS/Odu). Injections were done either at birth (day 0) or day 1 (n-STZ). Another group was administered the same volume of STZ 2 weeks after birth (I-STZ), and the control group received an injection of citrate buffer only. We measured blood glucose, body weight, serum triglycerides and pocket probing depths for the three groups. We also examined the extent of gingivitis among the n-STZ, I-STZ and control groups, and monitored macrophage function. After 18 weeks, the I-STZ group had significantly higher levels of blood glucose (p<0.001) and serum triglycerides than the other two groups. At 18 weeks the I-STZ group showed significantly lower body weight than either the n-STZ animals or the controls (p<0.001). Pocket probing depths of the I-STZ group were slightly increased compared with the n-STZ (p<0.05) and control (p<0.05) groups. At 18 weeks, macrophage chemotaxis in 1 : 20 and 1 : 40 zymosan activated serum was significantly greater in I-STZ than in the control group (p<0.05). The same was true for superoxide anion production measured by stimulation with phorbol myristate acetate and Ca ionophore A 23187 (p<0.001). These findings suggest that n-STZ rats have resistance to STZ. Based on blood glucose, body weight, pocket probing depths and serum triglycerides in the n-STZ group, we concluded that STZ affects the immune system.

Effects of streptozotocin-induced diabetes in neonatal rats with naturally occurring gingivitis

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