The Phagocytes in Hemolymph of Halocynthia roretzi and Their Phagocytic Activity

概要

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The phagocytes in hemolymph of Halocynthia roretzi and their phagocytic activity in vitro were studied by electron microscopy. Hemocytes were ultrastructurally classified as follows : Large granular amebocytes (LGs), small granular amebocytes (SGs), eight types of vacuolated or vesicular cells, dense granular cells and lymphoid cells. The vacuolated or vesicular cells, V1-V8, were distinguished from each other by the size of vacuole and the nature of inclusions. The identification of phagocytic cells and possible targets were examined in vitro. Freshly collected hemolymph was incubated with latex beads (LB) of 1, 5, 26μm in diameter, SRBC, Escherichia coli or small pieces of tunic. Small granular amebocytes actively ingested all the particles except the 26μm-LB. They surrounded the tunic pieces and the 26μm-LB. LGs ingested only 1μm-LB. Their activity was, however, weaker than that of SGs. We could not find any phagocytosed particles in other hemocytes during a 30min incubation. Furthermore, the plasma factor(s) that activated the phagocytosis to SRBC, did not influence that to the LB.
社団法人日本動物学会の論文
1994-10-15