The Primary Structure of Hemoglobin D from the Aldabra Giant Tortoise, Geochelone gigantea(Biochemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• The complete primary structures of α^D-2- and β-globin of hemoglobin D (Hb D) from the Aldabra giant tortoise, Geochelone gigantea, have been constructed by amino acid sequencing analysis in assistance with nucleotide sequencing analysis of PCR fragments amplified using degenerate oligonucleotide primers. Using computer-assisted sequence comparisons, the α^D-2-globin shared a 92.0% sequence identity versus α^D-globin of Geochelone carbonaria, a 75.2% versus α^D-globin of Aves (Rhea americana) and a 62.4% versus α^A-globin of Hb A expressed in adult red blood cells of Geochelone gigantea. Additionally, judging from their primary structures, an identical β-globin was common to the two hemoglobin components, Hb A and Hb D. The α^D-2- and β-globin genes contained the three-exon and twointron configurations and showed the characteristic of all functional vertebrate hemoglobin genes except an abnormal GC dinucleotide instead of the invariant GT at the 5' end of the second intron sequence. The introns of α^D-2-globin gene were both small (224-bp/first intron, 227-bp/second intron) such that they were quite similar to those of adult α-type globins ; the β-globin gene has one small intron (approximately 130-bp) and one large intron (approximately 1590-bp). A phylogenetic tree constructed on primary structures of 7 α^D-globins from Reptilia (4 species of turtles, 2 species of squamates, and 1 species of sphenodontids) and two embryonic α-like globins from Aves (Gullus gullus) and Mammals (Homo sapiens) showed the following results : (1) α^D-globins except those of squamates were clustered, in which Sphenodon punctatus was a closer species to birds than turtles ; (2) separation of the α^A- and α^D-globin genes occurred approximately 250 million years ago after the embryonic α-type globin-genes (π' and ζ) first split off from the ancestor of α-type globin gene family.

• 社団法人日本動物学会の論文
• 2002-02-25

著者

• Shishikura Fumio

  Department Of Biology Nihon University School Of Medicine

関連論文

• The Phagocytes in Hemolymph of Halocynthia roretzi and Their Phagocytic Activity
• BIO-DEFENSE STRATEGIES CONDUCTED BY HEMOLYMPH OF THE ASCIDIAN, HALOCYNTHIA RORETZI
• A Comparative Study on Earthworm Hemoglobins : An Amino Acid Sequence Comparison of Monomer Globin Chains of Two Species, Pontodrilus matsushimensis and Pheretima communissima That Belong to the Family Megascolecidae
• AMBYSTOMA MEXICANUM (AXOLOTL, AMPHIBIA) HEMOGLOBIN : THE COMPLETE AMINO ACID SEQUENCES OF TWO TYPES OF α GLOBINS AND ONE β GLOBIN(Biochemistry,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• Leech Extracellular Hemoglobin : Two Globin Strains That are Akin to Vertebrate Hemoglobin α and β Chains
• The Primary Structure of Hemoglobin D from the Aldabra Giant Tortoise, Geochelone gigantea(Biochemistry)
• Amino Acid Sequence of the Monomer Subunit of the Extracellular Hemoglobin of the Earthworm, Pheretima hilgendorfi
• The Amino Acid Sequences of the α- and β-Globin Chains of Hemoglobin from the Aldabra Giant Tortoises, Geochelone gigantea(Biochemistry)(Origin and evolution of chordates)
• A Case of Orthologous Sequences of Hemocyanin Subunits for an Evolutionary Study of Horseshoe Crabs : Amino Acid Sequence Comparison of Immunologically Identical Subunits of Carcinoscorpius rotundicauda and Tachypleus tridentatus

スポンサーリンク