Leech Extracellular Hemoglobin : Two Globin Strains That are Akin to Vertebrate Hemoglobin α and β Chains

スポンサーリンク

概要

• 論文の詳細を見る

• Leech (Whitmania edentula, Haemadipsa zeylanica var. japonica and Erpobdella lineata) extracellular hemoglobins are basically composed of three constituent subunits, a dimer (D_1 and D_2 chains) and two monomers (M1 and M2 chanis). We isolated these four chains from respective species by a combination of reversed-phase chromatography on a Resource RPC column and gel-filtration on a Superdex 75 column. The apparent molecular masses of the four globin chains were estimated by SDS-PAGE analysis to be 13 kDa (M1), 16 kDa (M2 ; 19 kDa in its reduced form) and about 27 kDa for the dimer subunit (13 kDa for D_1 ; 15 kDa for D_2), regardless of the source. The amino (N)-terminal segments (21-30 residues) from twelve globin chains of the above three species were determined and aligned. It was found that the twelve sequences could be separated into two distinct globin groups A and B. This finding supports the original idea of "two globin strains in annelid hemoglobin", which was proposed without any evidence for leech hemoglobins. Comparing the sequences in the three classes of Annelida, Hirudinea, Oligochaeta and Polychaeta, we found two invariant amino acids, Cys and Trp, which are interposed by eleven amino acid residues. Furthermore, the globin chains belonging to strain A were readily discernible as they had three more invariants, Ser-13, Asp-16 and Trp-28, while the globin chains of strain B had two more invariants, Lys-12 and Arg-27. Consequently, we propose that each of the three classes of Annelida have two distinct groups of globin chains that are akin to vertebrate hemoglobin α and β chains.

• 社団法人日本動物学会の論文

著者

• Shishikura Fumio

  Department of Biology, Nihon University School of Medicine

• Ochiai T

  Department Of Biology Fukushima Medical University

• Ochiai Takehiko

  Fukushima Medical College

• Yamanaka Ikuo

  University Forest in Chiba, Faculty of Agriculture, The University of Tokyo

• Shishikura Fumio

  Department Of Biology Nihon University School Of Medicine

• Yamanaka I

  University Forest The University Of Tokyo University

関連論文

• The Phagocytes in Hemolymph of Halocynthia roretzi and Their Phagocytic Activity
• BIO-DEFENSE STRATEGIES CONDUCTED BY HEMOLYMPH OF THE ASCIDIAN, HALOCYNTHIA RORETZI
• AQUATIC ANIMAL EXPERIMENT FACILITY FOR SPACE ENVIRONMENT UTILIZATION RESEARCH : FUNDAMENTAL TESTS WITH XENOPUS LAEVIS(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• AQUATIC ANIMAL EXPERIMENT FACILITY FOR SACE UTILIZATION RESEARCH WITH SMALL FRESHWATER FISH AND AMPHIBIAN(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• A Comparative Study on Earthworm Hemoglobins : An Amino Acid Sequence Comparison of Monomer Globin Chains of Two Species, Pontodrilus matsushimensis and Pheretima communissima That Belong to the Family Megascolecidae
• ISOLATION AND CHARACTERIZATION OF FISH MYOGLOBIN FROM HOKI (MACRURONUS MAGELLANICUS)(Biochemistry,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
• Developmental Status of Aquatic Animal Experiment Facility, Aquatic Habitat (AQH), for International Space Station
• AMBYSTOMA MEXICANUM (AXOLOTL, AMPHIBIA) HEMOGLOBIN : THE COMPLETE AMINO ACID SEQUENCES OF TWO TYPES OF α GLOBINS AND ONE β GLOBIN(Biochemistry,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• YEAST FLAVOHEMOGLOBIN FROM CANDIDA NORVEGENSIS : A SPECTRAL ANALYSIS FOR THE REDUCTION WITH NADH(Biochemistry,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• THE STABILITY PROPERTIES OF YEAST FLAVOHEMOGLOBIN(Biochemistry,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• YEAST FLAVOHEMOGLOBIN : STABILITY AND SPECTRAL PROPERTIES WITH SPECIAL REFERENCE TO ITS FAD GROUP(Biochemistry)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Leech Extracellular Hemoglobin : Two Globin Strains That are Akin to Vertebrate Hemoglobin α and β Chains
• CHANGE OF BROWN ADIPOSE CELLS OF LAND LEECHES, HAEMADIPSA ZEYLANICA JAPONICA WITH TEMPERATURE IN SUMMER(Cell Biology and Morphology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
• STRUCTURE OF BROWN FAT CELLS OF THE LAND LEECHES, HAEMADIPSA ZEYLANICA VAR. JAPONICA IN SPRING(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
• BROWN ADIPOSE CELLS OF THE LAND LEECH, HAEMADIPSA ZEYLANICA JAPONICA IN SUMMER(Cell Biology and Morphology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
• Factors Causing Changes in Brown Fat Cells in the Land Leeches, Haemadipsa zeylanica var. japonica in Winter
• The Primary Structure of Hemoglobin D from the Aldabra Giant Tortoise, Geochelone gigantea(Biochemistry)
• Amino Acid Sequence of the Monomer Subunit of the Extracellular Hemoglobin of the Earthworm, Pheretima hilgendorfi
• The Amino Acid Sequences of the α- and β-Globin Chains of Hemoglobin from the Aldabra Giant Tortoises, Geochelone gigantea(Biochemistry)(Origin and evolution of chordates)
• A Case of Orthologous Sequences of Hemocyanin Subunits for an Evolutionary Study of Horseshoe Crabs : Amino Acid Sequence Comparison of Immunologically Identical Subunits of Carcinoscorpius rotundicauda and Tachypleus tridentatus

もっと見る 閉じる

スポンサーリンク