Hormonal Control of Meiosis Initiation in the Testis from Japanese Newt, Cynops pyrrhogaster
スポンサーリンク
概要
- 論文の詳細を見る
Meiosis is an event that occurs prerequisitely and specifically in gametogenesis.However, the mechanisms of conversion from mitosis ot meiosis are poorly understood. I will review the results so far obtained by us using newt testis as a model system, and discuss about the extrinsic mechanism(s) controlling the conversion from mitosis to meiosis. In the newt spermatogonia enter meiosis in the 8^<th> generation after 7 mitotic divisions. We developed organ and reaggregate culture systems with a chemically defined medium in which porcine follicle-stimulating hormone (pFSH) promotes spermatogonial proliferation and differentiation into primary spermatocytes. Human recombinant stem cell factor (RhSCF) in vitro stimulates the spermatogonial proliferation and progression to the 7^<th> generation, but not the differentiation into primary spermatocytes; instead they die of apoptosis. The reason why rhSCF does not stimulate meiosis entrance seems to be due to the low level expression of c-kit protein at the 7^<th> generation of spermatogonia. Ovine PRL induces apoptosis in the 7^<th> generation of spermatogonia in vivo and in vitro. Incubation of newts at low temperature causes spermatogonial apoptosis by the elevation of plasma PRL titer. In the absence of FSH in organ culture spermatogonia can progress until the 7^<th> generation, but the 8^<th> generation never appear due to the apoptosis. Altogether there seems to be a regulatory checkpoint for entrance into meiosis in the 7^<th> generation. Spermatogonia could circumvent the checkpoint by the influence of some factor(s) produced by Sertoli cells upon activation by FSH. Trial to isolate factor(s) responsible for the meiosis-initiation is now underway.
- 社団法人日本動物学会の論文
- 2004-01-25
著者
-
安部 眞一
熊大・理・生科
-
安部 眞一
Department Of Biological Science Faculty Of Science Kumamoto University
-
Abe Shin-ichi
Department of Biological Science, Faculty of Science, Kumamoto University
-
Abe Shin-ichi
Department Of Biological Science Faculty Of Science Kumamoto University
関連論文
- Xtr, a plural tudor domain-containing protein, coexists with FRGY2 both in cytoplasmic mRNP particle and germ plasm in Xenopus embryo : Its possible role in translational regulation of maternal mRNAs
- 軟X線顕微鏡で見たイモリ精子
- 1T06 イモリ精子のX線イメージング
- Hormonal Control of Meiosis Initiation in the Testis from Japanese Newt, Cynops pyrrhogaster
- 両生類精卵細胞形成の制御 (2000年度日本繁殖生物学会シンポジウム講演論文)
- 両生類における精子分化機構 (生殖細胞の発生と性分化) -- (第3部 生殖巣の構築と配偶子形成)
- 培養系におけるイモリ精原細胞の増殖と減数分裂の開始
- 培養系による両生類の精子分化
- The Phagocytes in Hemolymph of Halocynthia roretzi and Their Phagocytic Activity
- 発生-13-In vitroにおける精子形成
- Meiotic divisions and early-mid-spermiogenesis from cultured primary spermatocytes of Xenopus laevis
- 細胞培養による精子形成--培養皿の中で精子をつくる (精子の生物学)
- EFFECTS OF HUMAN RECOMBINANT EGF(EPIDERMAL GROWTH FACTOR) AND ERBB INHIBITORS ON SPERMATOGONIAL PROLIFERATION AND DIFFERENTIATION IN ORGAN CULTURE OF NEWT TESTICULAR FRAGMENTS
- EXPRESSION OF OCCLUDIN IN NEWT TESTIS(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- EFFECT OF ESTRADIOL-17BETA (E2) ON THE PROLIFERATION AND DIFFERENTIATION OF NEWT SPERMATOGONIA IN TESTICULAR ORGAN CULTURE(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- BIO-DEFENSE STRATEGIES CONDUCTED BY HEMOLYMPH OF THE ASCIDIAN, HALOCYNTHIA RORETZI
- Functional demonstration of the ability of a primary spermatogonium as a stem cell by tracing a single cell destiny in Xenopus laevis
- PHENOLOXIDASE RELEASED BY HEMOCYTES INTO THE PLASMA IN THE SOLITARY ASCIDIAN HALOCYNTHIA RORETZI(Physiology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- DETECTION OF PHENOLOXIDASE ACTIVITY IN THE HEMOLYMPH OF HALOCYNTHIA RORETZI AND VACUOLATED CELLS(Physiology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Study of Color Variation in the Solitary Ascidian Halocynthia roretzi, Collected in the Inland Sea of Japan(Animal Diversity and Evolution)
- STUDY ON WHITE MABOYA (HALOCYNTHIA RORETZI) IN THE INLAND SEA(Cell Biology and Morphology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- Mutual and Directional Allogeneic Cytotoxic Reaction of Hemocytes in the Solitary Ascidian Halocynthia roretzi Revealed by One-step Quantitative Fluorimetric Assay(Cell Biology)
- Common Cell - Surface Antigens Functioning in Self - Recognition Reactions by Both Somatic Cells and Gametes in the Solitary Ascidian Halocynthia roretzi
- BRDU-UPTAKE CELLS IN JUVENILE SOLITARY ASCIDIAN, HALOCYNTHIA RORETZI(Physiology)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- ACTIVATION OF THE 58-KDA INHIBITOR PRECURSOR OF Halocynthia roretzi(Biochemistry)(Proceeding of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- COMMON CELL SURFACE ANTIGENS FUNCTIONING IN BOTH THE ALLOGENEIC CYTOTOXIC REACTION AND FERTILIZATION IN THE ASCIDIAN Halocynthia roretzi(Physiology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- ELECTRON MICROSCOPIC OBSERVATION OF HEMOPOIETIC TISSUE OF YOUNG HALOCYNTHIA RORETZI(Developmental Biology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- IS THE 58 KDA PLASMA INHIBITOR PRODUCED BY SMALL-GRANULAR AMEBOCYTE AND LARGE-GRANULAR AMEBOCYTE OF Halocynthia roretzi?(Biochemistry)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan
- TWO SUBPOPULATIONS ARE RECOGNIZED IN VIRIFORM CELLS, A KIND OF TUNIC CELLS OF HALOCYNTHIA RORETZI, BY VIRIFORM CELL-SPECIFIC MONOCLONAL ANTIBODY(Cell Biology and Morphology)(Proceedings of the Seventieth Annual Meeting of the Zoological Society of Japan)
- VIRIFORM CELL SEEN AS A NATIVE TUNIC CELL OF HALOCYNTHIA RORETZI THROUGH ANALYSIS OFGENOMIC DNA(Cell Biology and Morphology)(Proceedings of the Sixty-Ninth Annual Meeting of the Zoological Society of Japan)
- THE NATURE OF MARKERS FOR RECOGNITION ON HEMOCYTES IN ALLOGENEIC PHAGOCYTOSIS OF THE ASCIDIAN, HALOCYNTHIA RORETZI(Physiology)Proceedings of the Sixty-Third Annual Meeting of the Zoologiacal Socistry of Japan
- DEXTRAN SULFATE ACCELERATES THE INHIBITORY ACTIVITY OF PLASMA-ENZYME INHIBITOR FROM HALOCYNTHIA RORETZI HEMOLYMPH.(Biochemistry)Proceedings of the Sixty-Third Annual Meeting of the Zoologiacal Socistry of Japan
- THE INHIBITORY MECHANISM TO TRYPSIN OF A 58 KDA INHIBITOR FROM THE HEMOLYMPH PLASMA OF HALOCYNTHIA RORETZI.(Biochemistry)Proceedings of the Sixty-Third Annual Meeting of the Zoologiacal Socistry of Japan
- THE PHAGOCYTIC ACTIVITY OF SMALL GRANULAR AMEBOCYTE OF HALOCYNTHIA RORETZI : Physiology
- STICKY SUBSTANCE IN THE EARTHWORM COELOMOCYTES : Physiology
- PURIFICATION OF PLASMA ENZYME FROM THE ASCIDIAN, HALOCYNTHIA RORETZI, HEMOLYMPH. AN IMPROVED METHOD : Biochemistry : Abstracts of papers presented at the 60th Annual Meeting of the Zoological Society of Japan
- PURIFICATION OF THE PLASMA AMIDOLYTIC ENZYME-INHIBITING SUBSTANCE IN THE HEMOLYMPH OF AN ASCIDIAN, HALOCYNTHIA RORETZI : Biochemistry : Abstracts of papers presented at the 60th Annual Meeting of the Zoological Society of Japan
- ROLES OF GRANULAR AMOEBOCYTES ON ADHESION/AGGREGATION OF HEMOLYMPH OF HALOCYNTHIA RORETZI : Physiology : Abstracts of papers presented at the 60th Annual Meeting of the Zoological Society of Japan
- Cloning of a cDNA for Xenopus prolactin receptor and its metamorphic expression profile
- Genetic Factors Affect the Number of Circulating Primordial Germ Cells in Early Chick Embryos
- EFFECT OF TEMPERATURE ON PROLIFERATION OF SPERMATOGONIA, THEIR DIFFERENTIATION INTO PRIMARY SPERMATOCYTES AND VIABILITY OF GERM CELLS IN ORGAN CULTURE OF NEWT TESTES FRAGMENTS
- ROLE OF ANDROGEN RECEPTOR IN SEX DIFFERENTIATION IN JAPANESE FLOUNDER (PARALICHTHYS OLIVACEUS)(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- HSP90β IS INVOLVED IN SIGNALING PROLACTIN-INDUCED APOPTOSIS IN NEWT TESTIS
- SEMI-PERMEABLE BARRIER IN NEWT TESTIS(Cell Biology and Morphology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- cDNA CLONING AND mRNA EXPRESSION OF TRANSFORMER-2 IN THE MEDAKA, ORYZIAS LATIPES(Developmental Biology,Abstracts of papers presented at the 74^ Annual Meeting of the Zoological Society of Japan)
- EFFECT OF HIGHLY PURIFIED FSH ON SPERMATOGONIAL PROLIFERATION AND DIFFERENTIATION IN ORGAN CULTURE OF NEWT TESTIS(Developmental Biology)(Proceedings of the Seventy-Third Annual Meeting of the Zoological Society of Japan)
- Relative amounts of basic nuclear proteins SP4 and SP5 in Xenopus laevis sperm correlate with gene copy number
- EXPRESSION OF NEUREGULIN IN THE TESTIS OF XENOPUS LAEVIS(Developmental Biology,Abstracts of papers presented at the 75^ Annual Meeting of the Zoological Society of Japan)
- Involvement of Xtr (Xenopus tudor repeat) in microtubule assembly around nucleus and karyokinesis during cleavage in Xenopus laevis
- Low Temperature Promotes Annexin V Expression in Newt Testis(Reproductive Biology)
- PROLIFERATION OF SPERMATOGONIA AND INITIATION OF MEIOSIS IN CULTURE OF NEWT TESTES : EFFECT OF FSH AND INSULIN
- IDENTIFICATION AND CHARACTERIZATION OF A NOVEL NEUREGULIN ISOFORM IN THE TESTIS OF XENOPUS LAEVIS(Developmental Biology,Abstracts of papers presented at the 76^ Annual Meeting of the Zoological Society of Japan)
- THE ROLE OF PROLACTIN RECEPTOR IN PROLACTIN-INDUCED APOPTOSIS IN NEWT SPERMATOGONIA
- Cloning of cDNA for newt WT1 and the differential expression during spermatogenesis of the Japanese newt, Cynops pyrrhogaster
- Cloning and Expression of a cDNA Encoding a Prolactin Receptor of the Japanese Red-Bellied Newt, Cynops pyrrhogaster(Endocrinology)
- Conversion from mitosis to meiosis : Morphology and expression of proliferating cell nuclear antigen (PCNA) and Dmc1 during newt spermatogenesis
- Newt RAD51 : Cloning of cDNA and analysis of gene expression during spermatogenesis
- MECHANISM OF MEIOSIS INITIATION DURING SPERMATOGENESIS
- ISOLATION OF MOLECULES WHICH INTERACT WITH HSP90b IN GERM CELL APOPTOSIS IN NEWT TESTIS
- Xtr, a plural tudor domain-containing protein, is involved in the translational regulation of maternal mRNA during oocyte maturation in Xenopus laevis
- HORMONAL CONTROL OF SPERMATOGENESIS IN CYNOPS PYRRHOGASTER IN VITRO