<SESSION>EFFECT OF ORGANICALLY BOUND TRITIUM (OBT) ON PRE-IMPLANTATION MOUSE EMBRYOS INVITRO
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概要
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Effect of organically bound tritium (OBT), such as tritiated thymidine and tritium-labeled amino acids, on mouse preimplantation embryos was examined in vitro. Mouse zygotes fertilized in vitro (BC3F_1 eggs x ICR sperm) were cultured in the media containing OBT in various concentrations up to the blastocyst stage. The LD_<50> in terms of tritium concentrations in the culture medium were determined by measuring tritium concentrations in the medium to inhibit 50% of embryos to form blastocyst in vitro. Tritium activities in the embryos were measured at various times during culture of embryos at LD_<50> concentration in order to estimate absorbed radiation dose in embryonic cells. The LD_<50> values obtained indicate that OBT could inhibit the embryonic development 1000 times more effectively than tritiated water (HTO). However, differences in LD_<50> values in terms of absorbed radiation dose between OBT and HTO is not so essential, and might be explained by localized spatial distribution of OBT within the cell.
- 核融合科学研究所の論文
著者
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Ohyama Harumi
National Institute Of Radiological Sciences Radiation Hazards Research Group
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Ohyama Harumi
Division Of Radiobiology And Biodosimetry National Institute Of Radiological Sciences
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Ohyama Harumi
Divs. Biol. And Radiation Health National Institute Of Radiological Sciences
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Yamada Takeshi
Divs. Biol. and Radiation Health, National Institute of Radiological Sciences
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Yamada T
Tottori Univ. Fac. Medicine Yonago Jpn
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- EFFECT OF ORGANICALLY BOUND TRITIUM (OBT) ON PRE-IMPLANTATION MOUSE EMBRYOS INVITRO
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