High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells : Rapid Purification and Characterization

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概要

• 論文の詳細を見る

• Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.

• 社団法人 日本農芸化学会の論文
• 2007-10-23

著者

• HATA Keishi

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• Hiwatashi Kazuyuki

  東北大学 農学研究科栄養学

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita Jpn

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Kikuchi Ken-Ichi

  Department of Engineering in Applied Chemistry, Akita University

• TAKAHASHI Saori

  Akita Research Institute of Food and Brewing

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• Hata Keishi

  Institute For Food & Brewing Akita Prefectural Agricultural Forestry And Fisheries Research Cent

• Kojima Hiroaki

  R & D Company Kobayashi Pharmaceutical Co. Ltd.

• Takahashi Saori

  Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Saori

  Akita Res. Inst. Of Food And Brewing

• Kikuchi Ken-ichi

  Department Of Engineering In Applied Chemistry Akita University

• Hori K

  Kao Co. Ltd. Tochigi Jpn

• Hori Kazuyuki

  The Central Research Laboratory Hitachi Ltd.

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Akita University

• Hata Keishi

  Akita Res. Inst. Of Food And Brewing

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Graduate School Of Engineering And Resource Science A

• Kikuchi Ken-ichi

  Department Of Engineering In Applied Chemistry Graduate School Of Engineering And Resource Science A

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Takahashi S

  Department Of Biology The University Of Tokyo

• Gotoh Takeshi

  Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science

• Takahashi Satoru

  Institute Of Basic Medical Sceinces And Center For Tara

• KIKUCHI KEN-ICHI

  Department of Chemical Engineering for Resources, Akita University

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