High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization
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概要
- 論文の詳細を見る
Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%.
- 社団法人 日本農芸化学会の論文
著者
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HATA Keishi
Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen
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Gotoh Takeshi
Department of Engineering in Applied Chemistry, Akita University
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Kikuchi Ken-Ichi
Department of Engineering in Applied Chemistry, Akita University
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Takahashi Saori
Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Cen
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Takahashi Saori
Akita Res. Inst. Of Food And Brewing
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Hata Keishi
Akita Res. Inst. Of Food And Brewing
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