Purification and Properties of Extracellular Carboxyl Proteinase Secreted by Candida pulcherrima

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概要

• 論文の詳細を見る

• An extracellular proteinase secreted by Candida pulcherrima KSY 188-5 was purified about 60-fold to electrophoretical homogeneity from its culture supernatant, by ammonium sulfate fractionation,anion-exchange chromatography, and gel filtration. The proteinase had a molecular weight of approximately 36,500 and an isoelectric point of pH 4.7. The enzyme had an optimum pH of around 2.5-3.5 for activity and 3.0-5.0 for stability. The optimum temperature was around 45℃ at pH 3.0. The enzyme showed a broad substrate specificity for a variety of proteins to hydrolyze casein, BSA, hemoglobin keratin, and collagen. Among several proteinase inhibitors, pepstatin A completely abolished the enzyme activity;indicating that the extracellular proteinase from C. pulcherrima KSY 188-5 was classified in the group of carboxyl proteinases.

• 社団法人日本農芸化学会の論文
• 1995-03-23

著者

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Kikuchi Ken-Ichi

  Department of Engineering in Applied Chemistry, Akita University

• Gotoh Takeshi

  Department Of Materials Engineering And Applied Chemistry Mining College Akita University

• Konno Hiroyuki

  Department Of Applied Biological Chemistry Faculty Of Agriculture

• KOIZUMI Takeo

  Department of Brewing and Fermentation, Tokyo University of Agriculture

• Koizumi Takeo

  Department Of Brewing And Fermentation Tokyo University Of Agriculture

• Kikuchi Ken-ichi

  Department Of Engineering In Applied Chemistry Akita University

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Akita University

• Kodama Kenkichi

  Laboratory of Kodama BreweryAkita Konno Shoten

• Konno Hiroshi

  Akita Konno Shoten

• Kakuta Toshitaka

  Department of Brewing and Fermentation, Tokyo University of Agriculture

• Nojiro Kikuo

  Department of Brewing and Fermentation, Tokyo University of Agriculture

• Nojiro Kikuo

  Department Of Brewing And Fermentation Tokyo University Of Agriculture

• Kakuta T

  Tokyo Univ. Agriculture Tokyo Jpn

• Gotoh Takeshi

  Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science

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