Molecular Cloning and Analysis of the 5'-Flanking Region of the Human Thiamin Pyrophosphokinase Gene
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概要
- 論文の詳細を見る
We cloned and analyzed the 5'-flanking region of the human thiamin pyrophosphokinase gene (hTPK1). Truncation analysis using transiently transfected HepG2 cells revealed the minimal region required for basal activity of the hTPK1 promoter, which was encoded in a sequence between -105 and +441 relative to the transcription start site. In an electrophoretic mobility shift assay using the nuclear extracts from HepG2 cells and the synthetic oligonucleotide containing the Sp1 site, specific DNA-protein complexes were identified. These findings indicate the importance of the Sp1 cis-element in regulating the hTPK1 gene expression.
- 日本ビタミン学会の論文
著者
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Akaji Kenichi
Department of Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicin
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KONNO HIROYUKI
Department of Surgery II, Hamamatsu University School of Medicine
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NISHINO Hoyoku
Department of Biochemistry, Kyoto Prefectural University of Medicine
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Konno Hiroyuki
Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Akaji Kenichi
Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Nishino Hoyoku
Department Of Biochemistry And Molecular Biology Graduate School Of Medical Science Kyoto Prefectura
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Konno Hiroyuki
Department Of Applied Biological Chemistry Faculty Of Agriculture
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NOSAKA Kazuto
Department of Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicin
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ONOZUKA Mari
Graduate School of Medical Science, Kyoto Prefectural University of Medicine
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ONOZUKA Mari
Department of Biochemistry and Molecular Biology, Kyoto Prefectural University of Medicine
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Onozuka Mari
Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Nosaka Kazuto
Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Nosaka Kazuto
Department Of Chemistry Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Akaji Kenichi
Department Of Chemistry Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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