Synthesis of Human C-Type Natriuretic Peptide 22 Using Chlorotrityl Resin and Tetrafluoroboric Acid Deprotection
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概要
- 論文の詳細を見る
Human C-type natriuretic peptide 22 (hCNP22), the third member of the natriuretic peptide family, was efficiently synthesized by Fmoc-based peptide chain construction on a 2-chlorotrityl (Clt) resin, followed by deprotection using tetrafluoroboric acid (HBF_4). The use of Clt resin was effective in suppressing racemization at the C-terminal cysteine residue caused by the base treatment for Fmoc-cleavage. The disulfide bond of hCNP22 was constructed using the silyl chloride-sulfoxide method to avoid oxidation at the Met residue. Using amino acid- and dipeptide-resin derivatives, the effects of bases, protecting groups and resin supports on the racemization at the C-terminal Cys residue were examined in detail.
- 公益社団法人日本薬学会の論文
- 1996-07-15
著者
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Akaji Kenichi
Department of Chemistry, Graduate School of Medical Science, Kyoto Prefectural University of Medicin
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Kiso Y
Department Of Medicinal Chemistry Kyoto Pharmaceutical University
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KISO Yoshiaki
Department of Medicinal Chemistry, Center for Frontier Research in Medicinal Science and 21st Centur
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Akaji K
Kyoto Pharmaceutical Univ. Kyoto Jpn
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Akaji Kenichi
Department Of Medicinal Chemistry Kyoto Pharmaceutical University
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FUJIWARA Yasuhiro
Kyoto Pharmaceutical University
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Fujiwara Y
Kyoto Pharmaceutical University
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Kiso Yoshiaki
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science 21st Century Coe
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FUJIWARA YOICHI
Department of Medicinal Chemistry, Kyoto Pharmaceutical University
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YOKOTANI Junichi
Department of Medicinal Chemistry, Kyoto Pharmaceutical University
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Yokotani Junichi
Department Of Medicinal Chemistry Kyoto Pharmaceutical University
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Akaji Kenichi
Department Of Chemistry Graduate School Of Medical Science Kyoto Prefectural University Of Medicine
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Akaji Kenichi
Department Of Medicinal Chemistry Graduate School Of Medical Science Kyoto Prefectural University Of
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KISO Yoshiaki
Department of Ecological Engineering, Toyohashi University of Technology, JAPAN.
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