Analysis of Inactivation of AcMNPV under Various Conditions by the ELVA Method
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概要
- 論文の詳細を見る
Inactivation of baculoviruses was examined under various conditions by ELVA, which enzymatically labels the virus envelope with radioisotopes and detects the labeled viruses by host cell-specific binding. When baculoviruses were incubated in a 50-ml culture tube, they rapidly lost infective ability by more than 75% of the initial titer in 2 h even at an insect cell cultivation temperature of 27 °C. Altering pH from neutral significantly decreased the virus titer. Repetition of freezing and thawing of baculovirus solutions decreased the virus titer, but the addition of DMSO and glycerol prevented inactivation to some extent.
- 社団法人 日本農芸化学会の論文
- 2008-07-23
著者
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Gotoh Takeshi
Department of Engineering in Applied Chemistry, Akita University
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Gotoh Takeshi
Dep. Of Engineering In Applied Chemistry Akita Univ.
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Kikuchi Ken-ichi
Dep. Of Engineering In Applied Chemistry Akita Univ.
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Ando Nozomi
Department Of Engineering In Applied Chemistry Akita University
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Ando Nozomi
Department Of Bioresource Engineering Faculty Of Agriculture Yamagata University
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Kikuchi Ken-ichi
Department Of Engineering In Applied Chemistry Akita University
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Gotoh Takeshi
Department Of Engineering In Applied Chemistry Akita University
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Gotoh Takeshi
Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science
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