Probabilistic Characterization for Baculovirus-Infected Insect Cells Destined to Synthesize Progeny Viruses and Recombinant Protein and to Die

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概要

• 論文の詳細を見る

• A mathematical model using the Weibull distribution that characterizes the fate of virus-infected insect cells to conduct viral replication and subsequent recombinant protein synthesis and finally to die due to the progress of viral infection is presented. This model also takes into account the degradation of synthesized protein due to intrinsic denaturation and/or proteolytic hydrolysis. Model parameters were determined from the literature and based on experimental data. The model agrees well with the experimental data from cultures under different infectious conditions. Model simulations of virus-infected insect cultures reveal the importance of the interaction of the cell density at time of infection and multiplicity of infection and the presence of the best time for harvesting.

• 社団法人 化学工学会の論文
• 2004-11-01

著者

• 後藤 健彦

  広島大学大学院工学研究科

• Gotoh Takeshi

  Department of Engineering in Applied Chemistry, Akita University

• Kikuchi Ken-Ichi

  Department of Engineering in Applied Chemistry, Akita University

• Gotoh T

  Department Of Materials-process Engineering And Applied Chemistry For Environments Akita University

• Gotoh Takeshi

  Department Of Materials Engineering And Applied Chemistry Akita University

• Gotoh Takeshi

  Department Of Materials And Applied Chemistry Mining College Akita University

• Kikuchi Ken-ichi

  Department Of Materials-process Engineering And Applied Chemistry For Environments Akita University

• Kikuchi Ken-ichi

  Department Of Engineering In Applied Chemistry Akita University

• Kikuchi Ken-ichi

  Department Of Materials And Applied Chemistry Mining College Akita University

• Kikuchi K

  Akita Univ. Akita

• Gotoh Takeshi

  Department Of Engineering In Applied Chemistry Akita University

• CHIBA Kunihiro

  Department of Materials-Process Engineering and Applied Chemistry for Environments, Akita University

• Chiba K

  Department Of Materials-process Engineering And Applied Chemistry For Environments Akita University

• Gotoh Takeshi

  Department Of Engineering And Applied Chemistry Graduate School Of Engineering And Resource Science

• KIKUCHI KEN-ICHI

  Department of Chemical Engineering for Resources, Akita University

関連論文

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• Prorenin Processing Enzyme (PPE) Produced by Baculovirus-Infected Sf-9 Insect Cells: PPE Is the Cysteine Protease Encoded in the AcMNPV Gene
• 材料・界面
• 材料・界面
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• Analysis of Inactivation of AcMNPV under Various Conditions by the ELVA Method
• Purification and Characterization of Aspartic Protease Derived from Sf9 Insect Cells
• High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells: Rapid Purification and Characterization
• SEPARATION OF AMINO ACIDS BY ELECTRODIALYSIS WITH IONEXCHANGE MEMBRANES
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• The Occurrence of Renin Inhibitor in Rice : Isolation, Identification, and Structure-Function Relationship
• High-Level Expression of Recombinant Active Human Renin in Sf-9 Cells : Rapid Purification and Characterization
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• Mass transfer between particles and liquid in solid-liquid two-phase upflow in vertical tubes.
• Average particle velocity in solid-liquid two-phase flow through vertical and horizontal tubes.
• Correlation of mass transfer coefficient between particles and liquid in liquid fluidized beds.
• Mass transfer between particles and liquid in dilute fluidized beds in the low Reynolds number region.
• Correlation of liquid-side mass transfer coefficient for single particles and fixed beds.
• Axial dispersion of liquid in liquid fluidized beds in the low Reynolds number region.
• Mass transfer between particles and liquid in solid-liquid two-phase upflow in the low-velocity region through vertical tubes.
• Mass transfer between particles and liquid in solid-liquid two-phase flow through horizontal tubes.

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