Characterization of Acid-Stable Glucose Isomerase from Streptomyces sp., and Development of Single-Step Processes for High-Fructose Corn Sweetener (HFCS) Production

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概要

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• The glucose isomerase from Streptomyces olivaceoviridis E-86 was purified by chromatographic procedures, showing one single protein band in the SDS-PAGE. The enzyme had high acid stability, and there was no loss in enzyme activity at pH 5.0 after incubation at 60℃ for 30 hr. The enzyme had sufficients activity at 60℃, pH 5.5,(which is the reaction condition for a single-step process with a glucoamylase from A. niger), and at 58℃, pH 6.0,(condition with a glucoamylase from R. niveus). By using this acid-stable glucose isomerase, a single-step process to produce high-fructose corn sweetener (HFCS) from liquefied starch was formed without any reductant or other reagents for enzyme stabilization. The HFCS produced was about fifty percent fructose and less than 1.5% unknown oligosaccharides.

• 社団法人日本農芸化学会の論文
• 2000-05-23

著者

• Takahashi S

  Kyoto Univ. Kyoto Jpn

• Saito K

  Department Of Kampo Medicinal Science Hokkaido College Of Pharmacy

• Takahashi K

  School Of Life Sciences Tokyo Univ. Of Pharmacy And Life Sciences

• Takahashi Kayoko

  Central Research Lnstitute Meiji Milk Products Co. Ltd.

• Takahashi S

  Department Of Biosciences Teikyo University

• Takahashi S

  Akita Res. Inst. Of Food And Brewing

• Takahashi Saori

  Akita Res. Inst. Of Food And Brewing

• Takahashi S

  Akita Res. Inst. Food And Brewing Akita

• Takahashi Shunji

  Institute Of Molecular And Cellular Biosciences University Of Tokyo

• Takahashi Shunji

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• 斉藤 謙一

  Department Of Physiology And Anatomy Nihon University College Of Pharmacy

• TAKAHASHI Saori

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• SAITO Kyuichi

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• KANEKO Takahiro

  Department of Bioengineering, Akita Research Institute of Food and Brewing (ARIF)

• Saguchi Ken-ichi

  Laboratory Of Biopharmaceutics School Of Pharmaceutical Sciences Showa University

• Shinozaki Kazuo

  Riken Kanagawa Jpn

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Sasaki Kanako

  Riken Kanagawa Jpn

• Suzuki Takahito

  Biological Laboratory Faculty Of Science Nara Women's University

• Takahashi Saori

  Department Of Applied Biology Faculty Of Textile Science Kyoto Institute Of Technology

• Takahashi Kenji

  Laboratory Of Molecular Biochemistry School Of Life Science Tokyo University Of Pharmacy And Life Sc

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