Regulation Mechanism of the Serine Protease Activity of Plasma Hyaluronan Binding Protein
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概要
- 論文の詳細を見る
The inhibitor for the serine protease activity of plasma hyaluronan binding protein(PHBP)was purified from human plasma by polyethylene glycol(PEG)fractionation, diethylaminoethyl(DEAE)-Seohacel ion-exchange chromatography, Phenyl Toyopearl 650M hydrophobic chromatography, Bio Gel A-0.5m gel-filtration and hydroxyapatite chromatography. The serine protease activity of PHBP was measured with Boc-Phe-Ser-Arg-methycoumarine amide(MCA)as the synthetic substrate of PHBP. The results of the amino acid sequence analyses of the purified PHBP inhibitor indicated that it was C1 inhibitor of the serpin family. C1 inhibitor formed a complex with PHBP, suggesting that it is the actual inhibitor of PHBP in human plasma. On the other hand, dextran surface and phosphatidylethanolamine enhanced the auto-fragmentation and the serine protease actibity of pro-PHBP, but kaolin did not. These results suggested that the serine protease activity of PHBP was regulated in a similar manner to that of factor XII of the coagulation system.
- 公益社団法人日本薬学会の論文
- 2001-03-01
著者
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Choi-miura Nam-ho
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Miura-choi Nam-ho
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Saito K
Department Of Kampo Medicinal Science Hokkaido College Of Pharmacy
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Saito Kiyomi
Department of phamacology, showa University School of Medicine
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Saito Kiyomi
Department Of Phamacogenomics School Of Phamaceutical Sciences Showa University
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TOMITA Motowo
Department of Physiological Chemistry, School of Pharmaceutical Sciences Showa University
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Takahashi Katsuhiko
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
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YODA Madoka
Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University
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Takahashi Katsuhiko
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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斉藤 謙一
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
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冨田 昌弘
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Tomita Motowo
Department Of Hygienic Chemistry School Of Pharmaceutical Sciences Showa University
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Saito K
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
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Takahashi K
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Saguchi Ken-ichi
Laboratory Of Biopharmaceutics School Of Pharmaceutical Sciences Showa University
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Yoda Madoka
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Shinozaki Kazuo
Riken Kanagawa Jpn
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Sasaki Kanako
Riken Kanagawa Jpn
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Takahashi Katsuhiko
Department Of Artificial Complex Systems Engineering
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Takahashi Katsuhiko
Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology
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