Proteolytic Activation and Inactivation of the Serine Protease Activity of Plasma Hyaluronan Binding Protein

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概要

• 論文の詳細を見る

• We prepared anti-plasma hyaluronan binding protein (PHBP) mouse monoclonal antibodies and studied the fragmentation profile of PHBP with them. PHBP is present in human plasma as a single polypeptide chain (70kDa). During the purification, PHBP partially fragmentated into the 50-kDa N-terminal fragment and the 27-kDa C-terminal fragment. After the incubation of the purified PHBP, the 70-kDa precursor form was completely cleaved to the 50- and 27-kDa fragments, followed by the 50-kDa to the 26-kDa, and the 27-kDa to the 17-kDa plus the 8-kDa fragments, respectively. Because the purified PHBP contained no other detectable proteins and PHBP has a typical serine protease domain, we concluded that the fragmentation of PHBP was caused by own serine protease activity. PHBP cleaved the C-terminal side of Arg in the peptide effectively and that of Lysweakly. The results of the pre-incubation experiments of PHBP suggested that the single-chain form of PHBP is a precursor, the two-subunit structure is an active form and the three- or four-chain structure is an inactive form of a serine protease.

• 公益社団法人日本薬学会の論文
• 2001-05-01

著者

• Choi-miura Nam-ho

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• Saito K

  Department Of Kampo Medicinal Science Hokkaido College Of Pharmacy

• Saito Kiyomi

  Department of phamacology, showa University School of Medicine

• Saito Kiyomi

  Department Of Phamacogenomics School Of Phamaceutical Sciences Showa University

• TOMITA Motowo

  Department of Physiological Chemistry, School of Pharmaceutical Sciences Showa University

• Takahashi Katsuhiko

  Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University

• YODA Madoka

  Department of Physiological Chemistry, School of Pharmaceutical Sciences, Showa University

• MAZDA Toshio

  Japanese Red Cross Tokyo Blood Center

• Takahashi Katsuhiko

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• Choi‐miura N‐h

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• 斉藤 謙一

  Department Of Physiology And Anatomy Nihon University College Of Pharmacy

• 冨田 昌弘

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• Tomita Motowo

  Department Of Hygienic Chemistry School Of Pharmaceutical Sciences Showa University

• Mazda T

  Japanese Red Cross Tokyo Blood Center

• Saito K

  Department Of Physiology And Anatomy Nihon University College Of Pharmacy

• Takahashi K

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• Saguchi Ken-ichi

  Laboratory Of Biopharmaceutics School Of Pharmaceutical Sciences Showa University

• Yoda Madoka

  Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University

• Shinozaki Kazuo

  Riken Kanagawa Jpn

• Sasaki Kanako

  Riken Kanagawa Jpn

• Takahashi Katsuhiko

  Department Of Artificial Complex Systems Engineering

• Takahashi Katsuhiko

  Department of Applied Chemistry for Resources, Tokyo University of Agriculture and Technology

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