Quantitative Measurement of the Novel Human Plasma Protein, IHRP, by Sandwich ELISA
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概要
- 論文の詳細を見る
Inter-alpha-trypsin inhibitor family heavy chain-related protein(IHRP), which has a sequence similarity to the heavy chains of the inter-alpha-trypsin inhibitor(ITI)family, is a novel glycoprotein found in human plasma. We prepared two clones(1A4 and 6E11)of anti-IHRP mouse monoclonal antibody. Both of them recognized the C-terminal 35-kDa fragment which was produced by plasma kallikrein-degestion of IHRP. We developed a sandwich ELISA for measurement of the plasma IHRP concentration with the monoclonal antibody coated microtiter plate and the anti-N-terminal 57-kDa fragment of IHRP rabbit polyclonal antibody (anti-GP57). We found that the average concentration IHRP in the plasma of healthy donors was 101.3±31.8μg/ml(average±S.D.). The IHRP concentration in theplasma of patients with inflammatory disorders was slightly increased(137.5±40.2μg/ml : average±S.D.). Together with the previous data indicating the induction of the porcine mRNA, which is thought t be the species conterpart of human IHRP mRNA, in the liver after resuscitatio from cardiogenic shock, we propose that IHRP is a member of the acute-phase protein family.
- 公益社団法人日本薬学会の論文
- 2001-03-01
著者
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Choi-miura Nam-ho
Department Of Physiological Chemistry School Of Pharmaceutical Sciences Showa University
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Miura-choi Ho-nam
Department Of Physiological Chemistry School Of Pneumaceutical Sciences Showa University
関連論文
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- Isolation and Characterization of GBP28, a Novel Gelatin-Binding Protein Purified from Human Plasma
- Purification and Characterization of a Novel Hyaluronan-Binding Protein (PHBP) from Human Plasma: It Has three EGF, a Kringle and a Serine Protease Domain, Similar to Hepatocyte Growth Factor Activator
- Primary Structure of the Pig Homologue of Human IHRP: Inter-α-Trypsin Inhibitor Family Heavy Chain-Related Protein
- Regulation Mechanism of the Serine Protease Activity of Plasma Hyaluronan Binding Protein
- SP-40, 40 Is a Component of Plasminogen Activator Inhibitor-1-Binding Protein and Stabilizes Plasminogen Activator Inhibitor-1 Activity
- Hepatic Injury-Specific Conversion of Mouse Plasma Hyaluronan Binding Protein to the Active Hetero-Dimer Form
- Proteolytic Activation and Inactivation of the Serine Protease Activity of Plasma Hyaluronan Binding Protein
- Identification of the Substrates for Plasma Hyaluronan Binding Protein
- Quantitative Measurement of the Novel Human Plasma Protein, IHRP, by Sandwich ELISA