Effects of Dexamethasone and Aminophylline on Survival of Jurkat and HL-60 Cells(Pharmacology)
スポンサーリンク
概要
- 論文の詳細を見る
The effects of dexamethasone and aminophylline on survival of Jurkat T-lymphocytic leukemia cells and HL-60 promyelocytic leukemia cells were investigated. Dexamethasone (10, 1000nM) and aminophylline (1, 100μM) induced apoptosis in Jurkat and HL-60 cells in a concentration-dependent manner. Treatment with a combination of dexamethasone (10nM) and aminophylline (1μM) significantly increased the number of apoptotic HL-60 cells, but not that of Jurkat cells, compared with dexamethasone (10nM) or aminophylline (1μM) treatment alone. Dexamethasone and aminophylline also increased the number of phospho-histone H2B (Ser^<14>)-positive Jurkat and HL-60 cells. Phospho-histone H2B (pH2B)-positive HL-60 cells were significantly increased by treatment with a combination of dexamethasone (10nM) and aminophylline (1μM), although no such effect was observed in Jurkat cells. On the other hand, simultaneous treatment with 10nM dexamethasone and 1μM aminophylline activated the 36-kDa MBP kinase, pro-apoptotic protein kinase in HL-60 cells. The activation of 36-kDa MBP kinase by dexamethasone and aminophylline was supported by studies showing an increase in the number of pH2B-positive and apoptotic Jurkat and HL-60 cells upon exposure to these drugs. Thus treatment with a combination of dexamethasone and aminophylline accelerates apoptosis of HL-60 cells via activation of 36-kDa MBP kinase and H2B phosphorylation.
- 公益社団法人日本薬学会の論文
- 2006-02-01
著者
-
斉藤 謙一
北海道薬科大学
-
MASUKO Takashi
Department of Cell Biology, School of Pharmaceutical Sciences, Kinki University
-
Saito K
Department Of Kampo Medicinal Science Hokkaido College Of Pharmacy
-
Masuko Takashi
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
-
Kusama T
Laboratory Of Physiology And Anatomy College Of Pharmacy Nihon University
-
Kusama Tadashi
School Of Pharmacy Nihon Univ.
-
Kusama Tadashi
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
-
MASUKO Takashi
College of Pharmacy, Nihon University
-
KIZAWA Yasuo
Department of Physiology and Anatomy, Nihon University College of Pharmacy
-
FURUYA Minori
Department of Physiology and Anatomy, Nihon University College of Pharmacy
-
SAITO Kiyoshige
Department of Physiology and Anatomy, Nihon University College of Pharmacy
-
Kizawa Yasuo
School Of Pharmacy Nihon Univ.
-
Kizawa Yasuo
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
-
Kizawa Yasuo
Department Of Chemical Pharmacology Toho University School Of Pharmaceutical Sciences
-
斉藤 謙一
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
-
Saito K
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
-
Furuya Minori
Department Of Physiology And Anatomy Nihon University College Of Pharmacy
-
Furuya Minoru
Department Of Rehabilitation Tokyo Police Hospital
-
Saguchi Ken-ichi
Laboratory Of Biopharmaceutics School Of Pharmaceutical Sciences Showa University
-
Shinozaki Kazuo
Riken Kanagawa Jpn
-
Sasaki Kanako
Riken Kanagawa Jpn
-
Saito Ken-ichi
Department Of Toxicology And Pharmacology Faculty Of Pharmaceutical Sciences The University Of Tokyo
-
Sakoda K
Department Of Analytical Chemistry Faculty Of Pharmaceutical Sciences Hoshi University
-
Furuya Minoru
Department of Physiology and Anatomy, Nihon University College of Pharmacy
-
Saito Kiyoshige
Department of Chemical Pharmacology, Toho University School of Pharmaceutical Sciences
関連論文
- Polyacetylene Compounds from Atractylodes Rhizome
- A New Polyacetylene Compound from Atractylodes Rhizome
- Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation(Pharmacology)
- 細辛精油 methyleugenol の吸収と排泄 : 呼吸器
- Cholesterol Biosynthesis Inhibitory Component from Zingiber officinale ROSCOE
- Antibody epitope peptides as potential inducers of IgG antibodies against CD98 oncoprotein
- Identification of cell proliferation-associated epitope on CD98 oncoprotein using phage display random peptide library
- Molecular Structural and Functional Characterization of Tumor Suppressive Anti-ErbB-2 Monoclonal Antibody by Phage Display System
- Effect of Oral Treatment of Perilla frutescens and Its Constituents on Type-I Allergy in Mice
- Immunohistochemical expression and pathogenesis of BLM in the human brain and visceral organs
- Phage Display Cloning and Characterization of Monoclonal Antibody Genes and Recombinant Fab Fragment against the CD98 Oncoprotein
- Colocalization of CP125/CD98 with Tropomyosin Isoforms at the Cell-Cell Adhesion Boundary^1
- Mouse Inter-Alpha-Trypsin Inhibitor Family Heavy Chain-Related Protein is an Acute Phase Protein Induced by Inflammation
- Regulation of Gelatin-Binding Protein 28 (GBP28) Gene Expression by C/EBP
- Cloning of the cDNA for a Mouse Homologue of Human PHBP : a Novel Hyaluronan-Binding Protein
- Isolation and Characterization of the Plasma Hyaluronan-Binding Protein (PHBP) Gene (HABP2)
- Isolation and Characterization of the Human Inter-α-Trypsin Inhibitor Family Heavy Chain-Related Protein (IHRP) Gene (ITIHL1)
- Effects of Kangen-karyu on Coagulation System and Platelet Aggregation in Mice
- Identification of Truncated Human Glutamate Transporter
- Characterization and In Vitro Cytotoxic Effect of Adriamycin-conjugated Monoclonal Antibody Prepared Against Breast Cancer Cell Line
- Characterization of A New Breast Cancer-Associated Antigen and Its Relationship to MUC1 and TAG-72 Antigens
- Characterization of Cell Surface Antigens Expressed in the HMA-1 Breast Cancer Cell Line
- Enzyme Inhibitory Activities of Acetylene and Sesquiterpene Compounds in Atractylodes Rhizome
- Effects of Dexamethasone and Aminophylline on Survival of Jurkat and HL-60 Cells(Pharmacology)
- Malate dehydrogenases from nitrifying bacteria : purification and properties
- Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase from a Nitrite-Oxidizing Chemoautotroph, Nitrobacter agilis ATCC 14123 : Purification and Properties
- Regulation Mechanism of the Serine Protease Activity of Plasma Hyaluronan Binding Protein
- ラット及びヒトへの葛根湯(Ge-Gen-Tang)経口投与による尿中排泄成分について
- Urinary and Biliary Metabolites of Puerarin in Rats
- ラットへの葛根主要成分経口投与による体内動態
- Urinary and Biliary Metabolites of Daidzin and Daidzein in Rats
- Pharmacological Properties of Traditional Medicines. XXII. Pharmacokinetic Study of Mulberroside A and Its Metabolites in Rat
- Pharmacological Properties of Traditional Medicines (XXI) : Analysis of Plasma, Urine and Bile of Rats after Oral Administration of Water Extract of Mori Cortex
- Inhibitory Effects of Hydrolyzable Tannins form Melastoma dodecandrum LOUR. on Nitric Oxide Production by a Murine Macrophage-Like Cell Line, RAW264.7,Activated with Lipopolysaccharide and Interferon-γ
- Efficient Production of 2-Deoxyribose 5-Phosphate from Glucose and Acetaldehyde by Coupling of the Alcoholic Fermentation System of Baker's Yeast and Deoxyriboaldolase-Expressing Escherichia coli
- Microbial Production of 2-Deoxyribose 5-Phosphate from Acetaldehyde and Triosephosphate for the Synthesis of 2'-Deoxyribonucleosides(Microbiology & Fermentation Technology)
- Identificaion of a Domain Conferring Nucleotide Binding to the N-Acetyl-D-Glucosamine 2-Epimerase(Renin Binding Protein)
- Effects of Nucleotides on N-Acetyl-D-Glucosamine 2-Epimerases (Renin-Binding Proteins) : Comparative Biochemical Studies
- Serine Acetyltransferase Involved in Cysteine Biosynthesis from Spinach : Molecular Cloning, Characterization and Expression Analysis of cDNA Encoding a Plastidic Isoform
- Pharmacological Properties of Traditional Medicine (XXIX) : Effect of Hange-shashin-to and the Combinations of Its Herbal Constituents on Rat Experimental Colitis(Pharmacognosy)
- Anti-stress Effects of 3,4,5-Trimethoxycinnamic Acid, an Active Constituent of Roots of Polygala tenuifolia (Onji)(Pharmacognosy)
- Interaction between 3,4,5-trimethoxycinnamic acid and spinosin on pentobarbital-induced sleeping time in mice
- Hepatic Injury-Specific Conversion of Mouse Plasma Hyaluronan Binding Protein to the Active Hetero-Dimer Form
- Molecular Cloning of Acid-Stable Glucose Isomerase Gene from Streptomyces olivaceoviridis E-86 by a Simple Two-Step PCR Method, and Its Expression in Escherichia coli
- Proteolytic Activation and Inactivation of the Serine Protease Activity of Plasma Hyaluronan Binding Protein
- ESR Imaging on a Solid-tumor-bearing Mouse Using Spin-labeled Dextran
- Identification of Functionally Important Cysteine Residues of the Haman Renin-Binding Protein as the Enzyme N-Acetyl-D-Glucosamine 2-Epimerase 1
- Identification of the Substrates for Plasma Hyaluronan Binding Protein
- Renin Inhibits N-Acetyl-D-Glucosamine 2-Epimerase (Renin-Binding Protein)^1
- 漢方方剤「排膿散」中の枳実および芍薬成分(naringin,neohesperidin,paeoniflorin)の定量
- 生薬製剤の薬物学的特性-4-黄色ブドウ球菌226株によるマウスの膿瘍に対する排膿湯および排膿散の作用
- Evidence for the AT_1 Subtype of the Angiotensin II Receptor in the Rat Submandibular Gland
- Activation of Protein Kinase C Induces Internalization of the GABA_C Receptors Expressed in Xenopus Oocytes
- Homotypic Adhesion through Carcinoembryonic Antigen Plays a Role in Hepatic Metastasis Development
- MOLECULAR BIOLOGICAL STUDY ON SERINE BIOSYNTHETIC ENZYMES IN THE PHOSPHORYLATED PATHWAY FROM Arabisopsis thaliana
- Identification of Cysteine-380 as the Essential Residue for the Human N-Acetyl-D-Glucosamine 2-Epimerase (Renin Binding Protein)
- SUSCEPTIBILITY OF ANIMALS TO HEPATOCARCINOGENIC AROMATIC AMINES CORRELATES WITH THE INDUCTION OF THE CARCINOGEN ACTIVATION ENZYME (S) WITH THE AMINES
- Simultaneous Doping Analysis of Main Urinary Metabolites of Anabolic Steroids in Horse by Ion-trap Gas Chromatography-Tandem Mass Spectrometry
- Characterization and Quantification of Fluoxymesterone Metabolite in Horse Urine by Gas Chromatography/Mass Spectrometry
- Stir bar sorptive extraction with in situ derivatization and thermal desorption-gas chromatography-mass spectrometry for measurement of phenolic xenoestrogens in human urine samples
- Cloning and Characterization of cDNA for Inter-α-Trypsin Inhibitor Family Heavy Chain-Related Protein (IHRP), a Novel Human Plasma Glycoprotein
- Spatiotemporal ESR-CT Study on the Metabolism of Spin-labeled Polysaccharide in a Mouse
- Stopped-Flow and Spectrophotometric Study on Radical Scavenging by Tea Catechins and the Model Compounds
- Beta Ray-induced Scission of DNA in Tritiated Water and Protection by a Green Tea Percolate and (-)-Epigallocatechin Gallate
- Release of Nitric Oxide from a Spin Trap, N-tert-butyl-α-phenylnitrone, under Various Oxidative Conditions
- A Spin Trap, N-tert-Butyl-α-phenylnitrone Extends the Life Span of Mice
- Intracellular Localization of UDP-Glucuronosyltransferase Expressed from the Transfected cDNA in Cultured Cells
- Production of Plant Non-protein Amino Acids by Recombinant Enzymes of Sequential Biosynthetic Reactions in Bacteria
- Effects of the Acetylene Compound from Atractylodes Rhizome on Experimental Gastric Ulcers Induced by Active Oxygen Species
- Development of Miniaturized Hollow-fiber Assisted Liquid-phase Microextraction with in situ Acyl Derivatization Followed by GC-MS for the Determination of Benzophenones in Human Urine Samples
- Measurement of Benzophenones in Human Urine Samples by Stir Bar Sorptive Extraction and Thermal Desorption-Gas Chromatography-Mass Spectrometry
- Determination of Benzophenones in River-water Samples Using Drop-based Liquid Phase Microextraction Coupled with Gas Chromatography/Mass Spectrometry
- Analysis of glutathione and glutathione disulfide in human saliva using hydrophilic interaction chromatography with mass spectrometry
- Pharmacological Properties of Traditional Medicines 23.Searching for Active Compounds in the Blood and Bile of Rats after Oral Administrations of Extracts of Sansohnin(酸棗仁)
- 29D-07 遠志と酸棗仁の複合効果に関する研究
- Characterization of Acid-Stable Glucose Isomerase from Streptomyces sp., and Development of Single-Step Processes for High-Fructose Corn Sweetener (HFCS) Production
- Significance of integrin αvβ5 and erbB3 in enhanced cell migration and liver metastasis of colon carcinomas stimulated by hepatocyte-derived heregulin
- Pharmacological Properties of Traditional Medicines(26)Effects of Sansohnin-to(酸棗仁湯)on Pentobarbital Sleep in Stressed Mice
- Hollow-fiber-supported liquid phase microextraction with in situ derivatization and gas chromatography-mass spectrometry for determination of chlorophenols in human urine samples
- Determination of urinary triclosan by stir bar sorptive extraction and thermal desorption-gas chromatography-mass spectrometry
- Chromatographic and mass spectrometric analysis of glutathione in biological samples
- Miniaturized hollow fiber assisted liquid-phase microextraction and gas chromatography-mass spectrometry for determination of benzophenone and derivates in human urine sample
- Miniaturized hollow fiber assisted liquid-phase microextraction with in situ derivatization and gas chromatography-mass spectrometry for analysis of bisphenol A in human urine sample
- Pharmacological Properties of Galenical Preparation-12-Components of Chinese Traditional Prescription"KANZOBUSITO"(甘草附子湯)in Rat Portal Blood after Oral Administration
- Highly Sensitive Reporter Gene Assay for Dioxins in Human Plasma by Using Cycloheximide as an Enhancer Substance
- 生薬製剤の薬物学的特性-7-マウス皮内膿瘍に対する排膿散構成生薬の作用
- 漢方エキス製剤の品質評価について(第8報) : 3D-HPLCによる白朮アセチレン化合物の比較分析
- Pharmacological Properties of Traditional Medicines (XXI) : Analysis of Plasma, Urine and Bile of Rats after Oral Administration of Water Extract of Mori Cortex
- Application of a novel large-volume injection method using a stomach-shaped inlet liner in capillary gas chromatographic trace analysis of dioxins in human milk and plasma
- Epigenetic Assessment of Environmental Chemicals Detected in Maternal Peripheral and Cord Blood Samples
- 菱の抗菌活性成分について
- Disease-dependent Differently Methylated Regions (D-DMRs) of DNA are Enriched on the X Chromosome in Uterine Leiomyoma
- 漢方方剤「排膿散」中の枳実および芍薬成分(naringin, neohesperidin, paeoniflorin)の定量
- 生薬製剤の薬物学的特性(第VII報) : マウス皮内膿瘍に対する排膿散構成生薬の作用
- 生薬製剤の薬物学的特性(第IV報) : 黄色ブドウ球菌226株によるマウスの膿瘍に対する排膿湯および排膿散の作用
- 漢方エキス製剤の品質評価について(第2報) : 「乾姜」中の[6]-shogaol
- 生薬製剤の薬物学的特性(第VIII報) : 多形核白血球遊走能に対する排膿湯,排膿散および桔梗の作用
- 漢方エキス製剤の品質評価について(第4報) : [6]-gingerolおよび[6]-shogaolの定量と移行率
- 生薬製剤の薬物学的特性(第XI報) : 高速液体クロマトグラフ法によるラット尿および胆汁中の粉防巳成分Tetrandrineの定性と定量
- 漢方エキス製剤の品質評価について(第1報) : 「生姜」中の6-gingerol