Enhancement of Veratridine-Induced Sodium Dynamics in NG108-15 Cells during Differentiation(Pharmacology)
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概要
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Developmental changes in dynamics of Na^+ were studied in neuroblastoma×glioma hybrid NG108-15 cells during differentiation which was induced by dibutyryl cAMP (Bt_2cAMP). Ratiometric Na^+ imaging with a Na^+-sensitive fluorescent dye SBFI (sodium-binding benzofuran isophthalate) revealed that the intracellular Na^+ concentration ([Na^+]_i) was not affected by the application of high K^+ (60mM) solution to either control or differentiated cells. When cells were exposed to 50μM veratridine (Vtd), an agonist of voltage-sensitive sodium channels (VSSCs), a significant increase in [Na^+]_i was observed in differentiated but not in undifferentiated cells. Calculated mean [Na^+]_i value increased from the basal 10.4 to 44.1 mM in response to 50μM Vtd. This Vtd response was reversibly inhibited by tetrodotoxin (TTX), a specific blocker for VSSCs, in a dose-dependent manner (IC_<50>=1 nM). It is suggested that VSSCs in NG108-15 cells are sensitive to TTX and Vtd and that the number of VSSCs increases during differentiation.
- 2006-04-01
著者
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IMANISHI Takashi
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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MATSUSHIMA Kayoko
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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KAWAGUCHI Akinori
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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WADA Tetsuyuki
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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MASUKO Takashi
Department of Cell Biology, School of Pharmaceutical Sciences, Kinki University
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YOSHIDA Shigeru
Department of Life Science, School of Science & Engineering, Kinki University
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ICHIDA Seiji
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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Ichida S
Kinki Univ. Osaka Jpn
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Ichida Seiji
Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University
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Masuko Takashi
Department Of Agricultural And Biological Chemistry College Of Bioresource Sciences Nihon University
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Wada Tetsuyuki
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Wada Tetsuyuki
Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University
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Wada Tetsuyuki
Faculty Of Pharmaceutical Sciences Kinki University
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Kawaguchi Akinori
Department Of Biological Chemistry School Of Pharmaceutical Sciences Kinki University
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Masuko Takashi
Department Of Cell Biology School Of Pharmaceutical Sciences Kinki University
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Ichida S
Department Of Biological Chemistry Faculty Of Pharmaceutical Sciences Kinki University
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Imanishi Takashi
Division Of Biochemistry Kinki University School Of Pharmacy
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Matsushima Kayoko
Division Of Biochemistry Kinki University School Of Pharmacy
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Yoshida Shigeru
Department Of Applied Chemistry Graduate Schhol Of Science And Engineering Tokyo Institute Of Technology And Crest Japan Science And Technology Corporation (jst)
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