Expression of the Human Multidrug Resistance Gene (MDR1) and Prognostic Correlation in Human Osteogenic Sarcoma
スポンサーリンク
概要
- 論文の詳細を見る
The overexpression of P-glycoprotein (P-Gp), encoded by the human multidrug resistance gene (MDR1), decreases lipophilic drug accumulation in multidrug resistant cells in vitro. It is still not clear whether P-Gp contribute to the problem of multidrug resistance (MDR) in osteogenic sarcoma (OS). We examined the MDR1 expression of 20 OS specimens (11 primary tumors, 10 xenografts, 1 overlapping), by Northern blotting and by the reverse transcriptase-polymerase chain reaction (RT-PCR), and evaluated the relationship between MDR1 expression and patient prognosis. RT-PCR revealed MDR1 expression in 9/20 OS; 5/11 primary tumors and 5/10 OS-xenografts; northern blotting revealed MDR1 expression in only 5/20 OS. One primary OS specimen and its corresponding xenograft had similar levels of MDR1 expression. All 20 patients with OS were treated with chemotherapeutic protocols including doxorubicin, cisplatin, methotrexate and ifosfamide. Eight of 9 OS-patients expressing MDR1 were resistant to chemotherapy and had a poor prognosis. The relationship between MDR1 expression and poor prognosis in the 20 OS-patients was significant (p<0.01). The results support the assumption that MDR1 expression is related to MDR in human OS.
- 東海大学の論文
著者
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IMANISHI Takashi
Department of Biological Chemistry, School of Pharmaceutical Sciences, Kinki University
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TAMAOKI Norikazu
Department of Pathology, School of Medicine, Tokai University
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Ueyama Y
Dep. Of Pathology Tokai Univ. School Of Medicine
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Ueyama Y
Tokai Univ. School Of Medicine Kanagawa Jpn
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Ueyama Y
Tokai Univ. School Of Medicine Isehara‐shi Jpn
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Ueyama Yoshito
Department Of Pathology Tokai University School Of Medicine
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Tamaoki Norikazu
Department Of Pathology Tokai University School Of Medicine
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Tamaki Norikazu
Central Institute For Experimental Animals
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Fukuda Hiroaki
Department Of Orthopaedics Tokai University School Of Medicine
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Fukuda Hiroaki
Department Of Orthopedic Surgery Tokai University School Of Medicine
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SUTO Ryuji
Department of Orthopedics, Tokai University School of Medicine
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ABE Yoshiyuki
Department of Pathology, Tokai University School of Medicine
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TAMAOKI Norikazu
Central Institute for Experimental Animals
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HIGAKI Shozo
Department of Orthopedic Surgery, Tokai University School of Medicine
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UEYAMA Yoshito
Kanagawa Academy of Science and Technology (KAST)
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NAKAMURA Masato
Kanagawa Academy of Science and Technology (KAST)
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IMAI Nozomu
Department of Orthopedic Surgery, Tokai University School of Medicine
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Imanishi Takashi
Department Of Orthopedic Surgery Tokai University School Of Medicine
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Nakamura M
Department Of Surgery Pathology Tokai University School Of Medicine
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Suto Ryuji
Department Of Orthopedic Surgery Tokai University School Of Medicine
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Imai Nozomu
Department Of Orthopedic Surgery Tokai University School Of Medicine
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Higaki Shozo
Department Of Orthopedic Surgery Tokai University School Of Medicine
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Higaki Shozo
Department Of Orthopaedic Surgery Teikyo University School Of Medicine
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Abe Yoshiyuki
Department Of Surgery Pathology Tokai University School Of Medicine
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Tamaoki N
Central Institute For Experimental Animals
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Tamaoki Norikazu
Department Of Pathology School Of Medicine Tokai University
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Umezawa Y
Department Of Dermatology Tokai University School Of Medicine
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Abe Yoshiyuki
Department Of Orthopaedic Surgery Tohoku University School Of Medicine
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Nakamura Masato
Department Of Pathology Tokai University
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Ueyama Yoshihito
Department Of Pathology Tokai University School Of Medicine
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IMAI Nozomu
Department of Orthopaedic Surgery, School of Medicine, Tokai University
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FUKUDA Hiroaki
Department of Orthopaedic Surgery, Tokai University
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