PCR Method for Genotyping and Zygosity-Testing of RasH2 Transgenic Mice

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概要

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• In short-term carcinogenicity testing using CB6F1-TgrasH2 mice, sibling nonTgrasH2 mice are used as a negative control. However, selection of TgrasH2 and nonTgrasH2 mice has been performed by PCR with only transgene specific primers by the conventional method. Therefore, the conventional method involves the risk of false negative results due to reaction failure, and contamination with TgrasH2 mice in the control mice group. Based on the nucleotide sequence information around the pre-integration site, we developed a genotyping method for distinguishing not only TgrasH2 mice (hemizygous for the Tg allele) but also nonTgrasH2 (homozygous for the nonTg allele) in a positive manner.

• 社団法人　日本実験動物学会の論文

著者

• Oishi Yumiko

  Department Of Cardiology The University Of Tokyo

• SUEMIZU Hiroshi

  Central Institute for Experimental Animals

• Hioki Kyoji

  Central Inst. For Experimental Animals

• Hioki K

  Central Inst. Experimental Animals Kanagawa

• Hioki K

  Central Inst. Of Experimental Animals Kawasaki Jpn

• Ohishi Yumiko

  東京大学 医学系研究科循環器内科

• Tamaoki N

  Central Inst. For Experimental Animals

• Tamaki Norikazu

  Central Institute For Experimental Animals

• Tani N

  The 6th Department Of Internal Medicine School Of Medicine Tokai University

• OHNISHI Yasuyuki

  Central Institute for Experimental Animals

• SOTOMARU Yusuke

  Central Institute for Experimental Animals

• TAMAOKI Norikazu

  Central Institute for Experimental Animals

• MARUYAMA Chika

  Central Institute for Experimental Animals

• Tamaoki Norikazu

  Central Inst. For Experimental Animals

• KITO MARUYAMA

  Central Institute for Experimental Animals

• OGURA Tomoyuki

  Central Institute for Experimental Animals

• Ohnishi Yasuyuki

  Central Inst. For Experimental Animals

• Sotomaru Yusuke

  Tokyo Univ. Agriculture Tokyo

• Ogura Tomoyuki

  Central Inst. For Experimental Animals

• Kito-Maruyama Chika

  Central Institute for Experimental Animals

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