In vivo Acquired Drug Resistance and Multidrug Resistance Gene (MDR1) Expression in the KB Carcinoma Cell Line Xenotransplanted in Nude Mice

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概要

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• We studied the correlation between in vivo responsiveness of KB xenografts to anticancer drugs and the expression level of the human multidrug resistance gene (MDR1) encoding P-Glycoprotein (P-Gp). We established KB xenografts (xeKB3-1 and xeKB8-5) by inoculating these in vitro lines into nude mice. The responsiveness was evaluated by an in vivo chemosensitivity assay (T/C; sensitive, <50%). Xenograft xeKB3-1 was sensitive to vincristine (VCR) (T/C, 48%), and xeKB8-5 was resistant to VCR (T/C, 72%). We selected a VCR-resistant variant (xeKB3-1-R, T/C, 76%) by treating xeKB3-1 with VCR (1.2 mg/kg, x3) in vivo. The MDR1 expression was evaluated by a semi-quantitative assay using reverse transcription-polymerase chain reaction. A MDR1 expression pattern in xeKB3-1 and xeKB8-5 in vivo was the same as that to KB3-1 and KB8-5 in vitro. The xenograft xeKB3-1-R expressed definitive but significantly lower levels of MDR1 than xeKB8-5. These results suggest that acquired drug resistance is related to minimally enhanced expression of the P-Gp protein/MDR1 gene in KB xenografts in vivo.

• 東海大学の論文

著者

• Nakamura Masato

  Department Of Cardiology Ohashi Medical Center Toho University

• UEYAMA Yoshito

  Department of Pathology, School of Medicine, Tokai University

• TAMAOKI Norikazu

  Department of Pathology, School of Medicine, Tokai University

• Ueyama Y

  Dep. Of Pathology Tokai Univ. School Of Medicine

• Ueyama Y

  Tokai Univ. School Of Medicine Kanagawa Jpn

• Ueyama Y

  Tokai Univ. School Of Medicine Isehara‐shi Jpn

• Ueyama Yoshito

  Department Of Pathology Tokai University School Of Medicine

• Tamaoki Norikazu

  Department Of Pathology Tokai University School Of Medicine

• Tamaki Norikazu

  Central Institute For Experimental Animals

• Ogata Toshiro

  Department Of Surgery Kurume University School Of Medicine

• Ogata Toshiro

  Department Of Pathology Tokai University School Of Medicine Kanagawa Academy Of Science And Technolo

• ABE Yoshiyuki

  Department of Pathology, Tokai University School of Medicine

• Ueyama Yoshito

  Department Of Pathology School Of Medicine Tokai University

• TAMAOKI Norikazu

  Central Institute for Experimental Animals

• SAEGUSA Rieko

  Department of Pathology, Tokai University School of Medicine Kanagawa Academy of Science and Technol

• Nakamura M

  Department Of Surgery Pathology Tokai University School Of Medicine

• Saegusa Rieko

  Department Of Pathology Tokai University School Of Medicine Kanagawa Academy Of Science And Technolo

• Abe Yoshiyuki

  Department Of Surgery Pathology Tokai University School Of Medicine

• Tamaoki N

  Central Institute For Experimental Animals

• Tamaoki Norikazu

  Department Of Pathology School Of Medicine Tokai University

• Nakamura Masato

  Department Of Anesthesiology Kinki University School Of Medicine

• Umezawa Y

  Department Of Dermatology Tokai University School Of Medicine

• Abe Yoshiyuki

  Department Of Orthopaedic Surgery Tohoku University School Of Medicine

• Nakamura Masato

  Department Of Anesthesiology Kaizuka City Hospital

• Nakamura Masato

  Department Of Pathology Tokai University

• Ueyama Yoshihito

  Department Of Pathology Tokai University School Of Medicine

• UEYAMA Yoshito

  Department of Dermatology, Division of Specialized Clinical Science, Tokai University School of Medicine

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