An anti-K-ras ribozyme suppresses oncogene expression and cell growth of human pancreatic cancer

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概要

• 論文の詳細を見る

• Hammerhead ribozymes are effective modulators of gene expression due to their simple structure, site-specific cleavage activity and catalytic potential. The K-ras oncogene is thought to play an important role in the growth of pancreatic cancer, because an activated (mutated) ras gene is found in approximately 90% of human pancreatic cancers. In this study, we designed a hammerhead ribozyme directed against K-ras mRNA at codon 25 [K-ras Rz (25)], and investigated its efficacy in a cultured human pancreatic carcinoma cell line, MIA PaCa-2. K-ras Rz (25) significantly reduced the cellular K-ras mRNA level when introduced into the MIA PaCa-2 cells. The ribozyme suppressed cell growth. K-ras Rz (25) appears capable of reversing the malignant phenotype in human pancreatic carcinoma cells.

• 東海大学の論文

著者

• Kijima Hiroshi

  Department of Pathology, Hirosaki University School of Medicine

• Nakamura Masato

  Department Of Cardiology Ohashi Medical Center Toho University

• MINE Tetsuya

  Department of Gastroenterology and Hepatology, Tokai University School of Medicine

• UEYAMA Yoshito

  Department of Pathology, School of Medicine, Tokai University

• Ueyama Y

  Dep. Of Pathology Tokai Univ. School Of Medicine

• Ueyama Y

  Tokai Univ. School Of Medicine Kanagawa Jpn

• Ueyama Y

  Tokai Univ. School Of Medicine Isehara‐shi Jpn

• Ueyama Yoshito

  Department Of Pathology Tokai University School Of Medicine

• Kijima Hiroshi

  東海大学 医学部内科学

• Kijima Hiroshi

  Department Of Pathology Tokai University School Of Medicine

• YAMAZAKI Hitoshi

  Department of Physics,Faculty of Science,Okayama University

• Kijima H

  Tokai Univ. School Of Medicine Kanagawa Jpn

• Mine T

  Department Of Gastroenterology Tokai University School Of Medicine

• Mine T

  Division Of Gastroenterology And Hepatology Department Of Internal Medicine University Of Tokai Scho

• Kawada Hiroshi

  Department Of Hematology/oncology Tokai University School Of Medicine

• Kijima Hiroshi

  Department Of Pathology Tokai University School Of Medecine

• SAKUMA Toshiyuki

  Department of Pathology, Tokai University School of Medicine

• NISHI Masatake

  Department of Pathology, Tokai University School of Medicine

• ABE Yoshiyuki

  Department of Pathology, Tokai University School of Medicine

• Ueyama Yoshito

  Department Of Pathology School Of Medicine Tokai University

• Yamazaki Hitoshi

  Department Of Ophthalmology Hirosaki University School Of Medicine

• Hirabayashi Kenichi

  Department Of Pathology Tokai University School Of Medicine

• Nakamura M

  Department Of Surgery Pathology Tokai University School Of Medicine

• Yamazaki H

  Department Of Surgery Pathology Tokai University School Of Medicine

• Abe Yoshiyuki

  Department Of Surgery Pathology Tokai University School Of Medicine

• Nishi Masatake

  Department Of Pathology Tokai University School Of Medicine

• Nakamura Masato

  Department Of Anesthesiology Kinki University School Of Medicine

• Sakuma T

  Department Of Pathology Tokai University School Of Medicine:department Of Gastroenterology Tokai Uni

• Sakuma Toshiyuki

  Department Of Electronic Engineering Faculty Of Engineering Shinshu University

• Umezawa Y

  Department Of Dermatology Tokai University School Of Medicine

• Abe Yoshiyuki

  Department Of Orthopaedic Surgery Tohoku University School Of Medicine

• Kijima Hiroshi

  Department Of Hematology/oncology Tokai University School Of Medicine

• Kawada Hiroshi

  Research Center For Genetic Engineering And Cell Transplantation

• Mine Tetsuya

  Department Of Gastroenterology And Hepatology Tokai University School Of Medicine

• Yamazaki Hitoshi

  Department Of Ophthalmology Hirosaki University Graduate School Of Medicine

• Nakamura Masato

  Department Of Anesthesiology Kaizuka City Hospital

• Nakamura Masato

  Department Of Pathology Tokai University

• Ueyama Yoshihito

  Department Of Pathology Tokai University School Of Medicine

• UEYAMA Yoshito

  Department of Dermatology, Division of Specialized Clinical Science, Tokai University School of Medicine

• SAKUMA Toshiyuki

  Department of Electronic Engineering , Faculty of Engineering, Shinshu University

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