マイクロサテライトマーカー選抜法を用いたEGFPコンジェニックNOD/Shi-scid IL2Rg[null](NOG)マウスの作製

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概要

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• Severely immunocompromised NOD/Shi-scid IL2Rg null (NOG) mice, which show higher engraftment efficiency, are useful as recipients in xenotransplantation studies. We generated a NOG-enhanced green fluorescent protein (EGFP) transgenic (Tg) mouse (NOG-EGFP) that was introduced the EGFP transgene from the C57BL/6-EGFP Tg mouse using the speed congenic method with a marker-assisted selection protocol (MASP). With this method, the selection of the male with the closest NOG strain type was repeated four times. When human cord blood CD34+ cells were transplanted into NOD/Shi-scid, NOG, and NOG-EGFP mice (N6), the engraftment efficiency of the NOG-EGFP mice was significantly higher than that of the NOD/Shi- scid mice and was similar to that of the NOG mice. These results suggest that the NOG-EGFP mice, which were generated using the congenic method with MASP, acquired the immunological properties of the NOG mice.

• 2008-09-12

著者

• SUEMIZU Hiroshi

  Central Institute for Experimental Animals

• ITO Mamoru

  Central Institute for Experimental Animals

• Kawai Kenji

  Central Research Laboratories Sankyo Co. Ltd.

• KOSAKA Kinori

  Central Institute for Experimental Animals

• OGURA Tomoyuki

  Central Institute for Experimental Animals

• Ito M

  Central Inst. For Experimental Animals

• Suemizu Hiroshi

  Biomedical Research Department Central Institute For Experimental Animals

• Ogura Tomoyuki

  Central Inst. For Experimental Animals

• YAGIHASHI Chie

  Central Institute for Experimental Animals

• MIZUSHIMA Tomoko

  Central Institute for Experimental Animals

• ETOH Tomoo

  Central Institute for Experimental Animals

• Kawai Kenji

  Central Institute For Experimental Animals

• Ito Mamoru

  Central Inst. For Experimental Animals Kawasaki Jpn

• 末水 洋志

  Central Institute for Experimental Animals

• 八木橋 千枝

  Central Institute for Experimental Animals

• 水島 友子

  Central Institute for Experimental Animals

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