Application of a New Convenience Gender Sorting Method for Mouse Spermatozoa to Mouse Reproductive Engineering Technology
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概要
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In this study, we attempted to apply new convenience gender sorting methods using sex-determining region Y (SRY) gene expression on Y spermatozoa to mice. Mouse spermatozoa labeled with Cy3-SRY antibody conjugate were used for intracytoplasmic sperm injection (ICSI). In addition, spermatozoa conjugated with SRY antibody were conjugated with magnetic beads (Mag) and were pulled to the bottom of the medium. The supernatant of the medium was used for in vitro fertilization (IVF). The rate of males reproduced by ICSI using the spermatozoa conjugated with Cy3-SRY antibody was 86.1%. The female proportion reproduced by IVF using the spermatozoa separated in the supernatant after Mag-SRY antibody conjugation was 67.3%. These gender sorting methods are effective for the reproduction of transgenic mice.
著者
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SUEMIZU Hiroshi
Central Institute for Experimental Animals
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Hashimoto Haruo
Central Inst. For Experimental Animals
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ETO Tomoo
Central Institute for Experimental Animals
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Ito Mamoru
Central Inst. For Experimental Animals Kawasaki Jpn
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HASHIMOTO Haruo
Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki, Kanagawa 210–0821, Japan
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ITO Mamoru
Central Institute for Experimental Animals, Kawasaki-ku, Kawasaki, Kanagawa 210–0821, Japan
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