Higher susceptibility of NOG mice to xenotransplanted tumors
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概要
- 論文の詳細を見る
The purpose of tumorigenicity testing, as applied not only to cell substrates used for viral vaccine manufacture but also stem cells used for cell-based therapy, is to discriminate between cells that have the capacity to form tumors and cells that do not. Therefore, tumorigenicity testing is essential in assessing the safety of these biological materials. Recently developed NOD/Shi-scid IL2Rg^<null> (NOG) mice have been shown to be superior to NOD/Shi-scid (SCID) mice for xenotransplantation of both normal and cancerous cells. To select a suitable mouse strain as a xenogenic host for tumorigenicity testing, we compared the susceptibility of NOG (T, B, and NK cell-defective), SLID (T and B cell-defective), and the traditionally used nude (T cell-defective) mice to tumor formation from xenotransplanted HeLa S3 cells. When 10^4 HeLa S3 cells were subcutaneously inoculated into the flanks of these mice, the tumor incidence on day 22 was 10/10 (100%) in NOG, 2/10 (20%) in SCID, and 0/10 (0%) in nude mice. The subcutaneous tumors formed reproducibly and semiquantitatively in a dose-dependent manner. Unexpectedly, half of the NOG mice (5/10) that had been inoculated with a mere 10^1 HeLa S3 cells formed progressively growing subcutaneous tumors on day 78. We confirmed that the engrafted tumors originated from inoculated HeLa S3 cells by immunohistochemical staining with anti-HLA antibodies. These data suggest that NOG mice may be the best choice as a suitable strain for testing tumorigenicity.
- 日本トキシコロジー学会の論文
- 2009-02-01
著者
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Machida Kazuhiko
Central Institute for Experimental Animals
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TSUCHIYA Toshie
Division of Medical Devices, National Institute of Health Sciences
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Machida Kazuhiko
Testing Services Department Central Institute For Experimental Animals
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Machida Kazuhiko
The Department Of Pathology Nippon Experimental Medical Research Institute Co. Ltd
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Ohnishi Yasuyuki
Biomedical Research Department Central Institute For Experimental Animals
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Sawada Rumi
Division Of Medical Devices National Institute Of Health Sciences
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Suemizu Hiroshi
Biomedical Research Department Central Institute For Experimental Animals
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Kawai Kenji
Pathology Research Department, Central Institute for Experimental Animals
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Ishikawa Tsuyoshi
Division of Medical Devices, National Institute of Health Sciences
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Ishikawa Tsuyoshi
Division Of Medical Devices National Institute Of Health Sciences
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Tsuchiya Toshie
Division Of Medical Chemistry National Institute Of Hygienic Sciences
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Kawai Kenji
Pathology Research Department Central Institute For Experimental Animals
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