ラット大腸における異常陰窩巣形成ならびにrasH2マウスにおける大腸発癌に対するヨーグルトの抑制効果

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概要

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• The inhibitory effects of yogurt consisting of milk fermented by Lactobacillus delbrueckii subsp. bulgaricus strain 2038 and Streptococcus salivarius subsp. thermophilus strain 1131 on formation of colonic aberrant crypt foci (ACF) in rats and also on development of colorectal tumors in transgenic mice harboring human prototype c-Ha-ras genes (rasH2 mice) were examined. F344 rats and rasH2 mice were fed commercial diet containing freeze-dried yogurt or starter medium (non-fermented milk). Rats were inoculated orally with heterocyclic amine 2-amino-methyl-6-phenylimidazo[4,5-b]pyridine hydrochloride (PhIP) for two weeks. The rats were necropsied 14 days after the PhIP treatment, and ACF in the colon and rectum were counted. RasH2 mice were injected with 1,2-dimethylhydrazine dihydrochloride (DMH) for 20 weeks. Three weeks after the last injection of DMH, rasH2 mice were necropsied to determine the number and the size of colorectal tumors. Yogurt supplementation in diet significantly reduced the number of ACF and aberrant crypts (ACs) in rats fed control diet (P<0.01), but not in rats fed non-fermented milk diet. On the other hand, rasH2 mice receiving the yogurt-supplemented diet had significantly reduced numbers of tumors induced by DMH compared with those fed the non-fermented milk-supplemented diet (P<0.05). These results demonstrate that the yogurt used in this study appears to have tumor-suppressing properties, and rasH2 mice are a useful model for the evaluation of antitumor activities of foods.

著者

• ITOH Kikuji

  Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Sciences, The Univer

• Hioki Kyoji

  Central Inst. For Experimental Animals

• Hioki Kyoji

  Central Institute For Experimental Animals

• Nomura Tatsuji

  Central Institute For Experimental Animals

• ITOH Toshio

  Central Institute for Experimental Animals

• NARUSHIMA Seiko

  Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Science, The Univers

• Itoh Kikuji

  Laboratory Of Veterinary Public Health

• SAKATA Tadashi

  Meiji Dairies Corporation

• Narushima Seiko

  Laboratory Of Veterinary Public Health Graduate School Of Agricultural And Life Sciences The Univers

• 成島 聖子

  Laboratory of Veterinary Public Health, The University of Tokyo

• 阪田 匡

  Meiji Dairies Corporation

• 日置 恭司

  Central Institute for Experimental Animals

• ITOH Kikuji

  Laboratory of Veterinary Public Health, The University of Tokyo

• NARUSHIMA Seiko

  Laboratory of Veterinary Public Health, The University of Tokyo

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