Conversion of the Genetic Background of Transgenic Mice by In Vitro Fertilization

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概要

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• We attempted to convert the genetic background of transgenic (Tg) mice in a short period of time by applying in vitro fertilization techniques. Tg mice were obtained by injecting human interleukin-2 (hIL-2) gene into the fertilized eggs of the C57BL/6N strain. These Tg mice were back-crossed 8 times to the inbred C3H/HeN strain using the hIL-2 gene as the genetic selection marker. In order to shorten the length of the back-crossing time, in vitro fertilization was performed with eggs collected from immature Tg females and spermatozoa from mature C3H/HeN males, and successfully fertilized eggs (2-cell stage) were transferred to pseudopregnant recipients to obtain the offspring of next generation. When no offspring were obtained through the procedures using immature Tg females, in vitro fertilization was performed with mature Tg males and mature C3H/HeN females to continue successive back-crosses. With this method, it was possible to perform 8 successive back-crosses in 18 months.

• 社団法人　日本実験動物学会の論文

著者

• YOKOYAMA Minesuke

  Mitsubishi Kagaku Institute of Life Sciences

• Nomura T

  Central Inst. For Experimental Animals

• Nomura Tatsuji

  実験動物中央研究所

• Nomura Tatsuji

  Central Institute For Experimental Animals

• YOKOYAMA MINESUKE

  Central Institute for Experimental Animals

• KATSUKI Motoya

  Medical Institute of Bioregulation, Kyushu Univ.

• HASEGAWA Takanori

  Nippon Syntex Niihari Research Center

• Katsuki Motoya

  Medical Institute Of Bioregulation Kyushu University

• Yokoyama Minesuke

  Department Of Pathology Nippon Medical School

• Katsuki M

  Center For Experimental Medicine Institute Of Medical Science University Of Tokyo

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