Simple and Efficient Vitrification Procedure for Cryopreservation of Mouse Embryos
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概要
- 論文の詳細を見る
Mouse pronuclear oocytes and 2-cell embryos derived from in vitro fertilization were cryopreserved by a novel simple vitrification procedure. Most cryopreserved oocytes/embryos were morphologically normal after warming, and 89-92% of them developed to the blastocyst stage during the culture. Moreover, the rate of morphologically normal pronuclear oocytes after being repeatedly cooled and warmed three times was as high as that of oocytes cooled and warmed only once, and 85% of them developed to the blastocyst stage. In addition, 43-57% of the cryopreserved oocytes/embryos transferred to recipients had developed normally to live fetuses observed on day 18.5 of pregnancy.
- 社団法人 日本実験動物学会の論文
著者
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Nakamura Naoko
Division Of Reproductive Engineering Center For Animal Resources And Development (card) Kumamoto Uni
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Nakagata N
Center For Animal Resources And Dev. Kumamoto Univ.
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Nakagata N
Kumamoto Univ. Kumamoto Jpn
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KATSUKI Motoya
Department of Molecular and Cellular Biology, Medical Institute of Bioregulation, Kyushu University
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Katsuki Motoya
Medical Institute Of Bioregulation Kyushu University
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Katsuki Motoya
Department Of Dna Biology School Of Medicine Tokai University:central Institute For Experimental Ani
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Nakao K
Univ. Tokyo Tokyo
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NAKAO Kazuki
Department of DNA Biology and Embryo Engineering, Institute of Medical Science, University of Tokyo
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NAKAGATA Naomi
Department of DNA Biology and Embryo Engineering, Institute of Medical Science, University of Tokyo
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Katsuki M
Center For Experimental Medicine Institute Of Medical Science University Of Tokyo
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Katsuki Motoya
Department Of Dna Biol. Embryo Engeneering Institute Of Medical Science University Of Tokyo
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