Establishment of Specific Pathogen-Free Guinea-pig Colonies Using Limited-Flora Guinea-pigs Associated with Conventional Guinea-pig Flora, and Monitoring of their Cecal Flora
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概要
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Six groups of limited flora (LF) Hartley guinea-pigs were produced by inoculation of hysterectomy-derived GF guinea-pigs with various combinations of cecal bacteria of conventional (CV) guinea-pigs to determine the effective bacterial cocktails for the establishment of a specific pathogen free (SPF) colony. Bifidobacterium magnum (Bif) isolated from CV guinea-pigs was used for pretreatment. The mortality of LF guinea-pigs inoculated with only Bif was 75%, and that of those inoculated with Bif plus chloroform-treated cecal suspension (CHF) or Bif plus CHF plus 32 isolates from CV guinea-pigs was 40 to 66.7%. These three groups were in an unhealthy condition with mucoid enteritis-like diarrhea. However, the mortality of LF guinea-pigs inoculated with the anaerobic growth on EG plates injected with 10-5 dilution of cecal contents (CF) or inoculated with Bif plus CF was 6.3 and 15%, respectively. These latter two groups of LF guinea-pigs were transferred to separate barrier rooms and some of the LF guinea-pigs were maintained in isolators as a source of intestinal flora for SPF guinea-pigs. The composition of cecal flora of LF guinea-pigs was stable for a long time, and bacteroidaceae and peptococcaceae were maintained as predominant components. The basic composition of the cecal flora of SPF guinea-pigs originated from LF guinea-pigs, which consists mainly of the anaerobic bacteria, was not changed over a long period, and the flora composition became similar to that in CV guinea-pigs. Guinea-pig-specific pathogens from the SPF colonies were not detected during experiments.
- 社団法人 日本実験動物学会の論文
著者
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Sudou K
Okayama Univ. Okayama Jpn
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ASAI Hidekazu
Japan SLC, Inc.
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GONDA Toshihiko
Japan SLC, Inc.
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YANABE Makoto
Japan SLC, Inc.
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SHIBUYA Masaharu
Japan SLC, Inc.
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TANAKA Toshio
Japan SLC, Inc.
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SUDOU Katuko
Institute of Medical Science, The University of Tokyo
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NARITA Teruo
Sankyo Co., Ltd.
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ITOH Kikuji
Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Sciences, The Univer
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MATSUI Takane
Department of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine
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Yanabe M
Japan Slc Inc. Shizuoka Jpn
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Sato Katsunori
Japan Slc Inc.
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Sato K
Faculty Of Agriculture Okayama University
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Matsui Takane
Department Of Veterinary Pathology Obihiro University Of Agriculture And Veterinary Medicine
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Matsui Takane
Department Of Comparative Pathology Faculty Of Veterinary Medicine Hokkaido University
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Itoh Kikuji
Lab. Of Veterinary Public Health The Univ. Of Tokyo
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Itoh Kikuji
Laboratory Of Veterinary Public Health Graduate School Of Agriculture And Life Science The Universit
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Narita T
Sankyo Co. Ltd.
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Asai Hidekazu
Japan Slc Inc.
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Narita Teruo
Sankyo Co. Ltd.
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Gonda Toshihiko
Japan Slc Inc.
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Shibuya Masaharu
Japan Slc Inc.
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Itoh Kikuji
Dep. Of Veterinary Public Health Graduate School Of Agricultural And Life Sciences The Univ. Of Toky
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Itoh Kikuji
Laboratory Of Veterinary Public Health
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Yanabe Makoto
Japan Slc Hamamatsu City
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Tanaka Toshio
Japan Slc Inc.
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Matsui Takane
Department Of Clinical Veterinary Science Obihiro University Of Agriculture And Veterinary Medicine
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