New PCR-RFLP Analysis for the Mouse Tnfsf6^<gld> Gene Caused by a Point Mutation in the Tnfsf6 [Tumor Necrosis Factor (Ligand) Superfamily, Member 6] Locus

概要

論文の詳細を見る
A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation.
社団法人　日本実験動物学会の論文
2002-10-01