A New Molecular Genetic Diagnosis of the Btkxid Mice

概要

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A new method of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of a point mutation of the Brutons tyrosine kinase (Btk) gene in CBA/N mice bearing an X-linked recessive immunodeficiency (xid). Since restriction site useful for RFLP analysis does not exist in the spontaneous mutant Btkxid locus, an artificial restriction site was introduced by PCR amplification with a modified primer. The five genotypes of the Btk locus (Btkxid/ Btkxid, Btkxid /Btk+ and Btk+/Btk+ females and Btkxid/Btknull and Btk+/Btknull males) could be distinguished by three patterns clearly and easily. This PCR-RFLP analytic method will be useful as a tool in the production of congenic mice and mice with multiple immunodeficient genes.
社団法人　日本実験動物学会の論文