A Rapid and Easy Electrophoretic Method for Detecting Biochemical Loci of Rat (Rattus norvegices)
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概要
- 論文の詳細を見る
Methods for electrophoresis for the analysis of biochemical marker genes, which are used widely for genetic monitoring of inbred straines of rats, have been complicated by the variation of the gel and electrode buffer and electrophoretic conditions with the enzyme or the protein to be examined. To simplify the methods, we performed electrophoresis under fixed conditions of 200 V and 30 min using cellulose acetate membrane as the gel and veronal solution as the gel and electrode buffer. Good results were obtained concerning 12 loci, namely, Amy1, Cs1, Esl, Es2, Es3, Es4, Fh1, Gc, Hbb, Ldr1, Mup1, and Svp1. This method was applied to 8 inbred strains of rats and confirmed to be practical.
- 社団法人 日本実験動物学会の論文
著者
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NISHIKAWA Tetsu
Institute for Experimental Animals, Hamamatsu University School of Medicine
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Nishikawa Tetsu
Institute For Experimental Animals Hamamatsu University School Of Medicine
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NISHIMURA Masahiko
Institute for Laboratory Animal Research, Nagoya University School of Medicine
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Nishimura Masahiko
Institute For Experimental Animals Hamamatsu University School Of Medicine
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