Identification and Minisatellite Linkage Analysis of SMXA Recombinant Inbred Strains of Mice by DNA Fingerprinting
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概要
- 論文の詳細を見る
SMXA recombinant inbred (RI) strains of mice were produced by systematic inbreeding from the F2 generation of a cross between two progenitor inbred strains, A/J and SM/J, which differ considerably with respect to many characteristics, and consists of 28 inbred strains. In this study, we investigated the applicability of DNA fingerprinting with M13 phage DNA to the identification of these closely related strains. DNA fingerprints of the SMXA RI strains and their progenitors, SM/J and A/J, showed strain-specific patterns, with the same banding patterns within each strain. Linkage analysis by using strain distribution patterns of minisatellite loci with 108 genetic markers containing microsatellites, biochemical and immunological marker genes allowed 23 minisatellite loci to be assigned to 11 chromosomes. The results suggested that DNA fingerprinting with M13 phage DNA is applicable not only for strain identification but also for genetic monitoring of RI strains on almost all chromosomes.
- 社団法人 日本実験動物学会の論文
著者
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MANNEN Hideyuki
Laboratory of Animal Breeding and Genetics, Graduate School of Agricultural Science, Kobe University
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NISHIMURA Masahiko
Institute for Laboratory Animal Research, Nagoya University School of Medicine
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Sudo Toshiki
Department Of Pharmacology And Experimental Therapeutics Tokushima Research Institute Otsuka Pharmac
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Nishimura M
Hamamatsu Univ. School Of Medicine Shizuoka Jpn
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Nishimura Masahiko
Institute For Experimental Animals Hamamatsu University School Of Medicine
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FUKUTA Katsuhiro
National Institute of Animal Health
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Mannen Hideyuki
Laboratory Of Animal Breeding And Genetics Faculty Of Agriculture Kobe University
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Fukuta Katsuhiro
Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ
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Fukuta Katsuhiro
Graduate School Of Bioagricultural Sciences Nagoya University
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TSUJI Soichi
Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University
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GOTO Nobuo
Laboratory of Animal Breeding and Genetics, Faculty of Agriculture, Kobe University
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Fukuta K
Nagoya Univ. Nagoya
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Fukuta Katsuhiro
Lab. Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ.
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Tsuji Soichi
Laboratory Of Animal Breeding And Genetics Faculty Of Agriculture Kobe University
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Goto Nobuo
Laboratory Of Animal Breeding And Genetics Faculty Of Agriculture Kobe University
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Sudo Toshiki
Department Of Cardiovascular Medicine Institute Of Health Biosciences The University Of Tokushima Gr
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