Isolation of a Germline-Transmissible Embryonic Stem (ES) Cell Line from C3H/He Mice

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概要

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• We have isolated three embryonic stem (ES) cell lines from C3H/He mice using mouse STO cells as a feeder layer. One ES cell line (H-1) was male, and two (H-2 and H-3) were female, as determined by polymerase chain reaction, in situ hybridization, and karyotype analyses. All were immunocytochemically reactive with a C3H strain-specific antibody. Injection of cells from the female ES H-3 line into C57BL/6 blastocysts yielded four chimeras with slight coat color chimerism. All chimeras were male, and as expected, no germline-transmission was observed. By contrast, when male ES H-1 cells were injected into the perivitelline space of 8-cell C57BL/6 embryos, one male mouse with overt coat color chimerism was recovered, and it produced ES H-1-derived offspring exclusively. This germline-transmissible C3H/He cell line represents a novel addition to those ES lines currently employed for gene manipulation studies of development.

• 社団法人日本動物学会の論文
• 1996-12-15

著者

• Kitani H

  National Inst. Animal Health Ibaraki Jpn

• Kitani Hiroshi

  National Institute Of Agrobiological Sciences

• IMAMURA Kenkichi

  National Institute of Animal Health

• GOTO Saori

  National Institute of Animal Health

• FUKUTA Katsuhiro

  National Institute of Animal Health

• Fukuta Katsuhiro

  Laboratory Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ

• Fukuta Katsuhiro

  Graduate School Of Bioagricultural Sciences Nagoya University

• Fukuta Katsuhiro

  Laboratory Of Animal Morphology And Function Graduate School Of Bioagricutural Sciences Nagoya Unive

• 高木 信夫

  北海道大学大学院地球環境科学研究科

• 高木 信夫

  北大・理・染色体研

• 高木 信夫

  北海道大学遺伝子実験施設

• 高木 信夫

  北大 地球環境科学

• Takagi N

  Division Of Bioscience Graduate School Of Environmental Earth Science Hokkaido University

• Takagi N

  Yokohama City Univ. Yokohama Jpn

• Imamura K

  National Institute Of Animal Health

• Fukuta K

  National Inst. Animal Health Ibaraki Jpn

• Fukuta K

  Nagoya Univ. Nagoya

• Fukuta Katsuhiro

  Lab. Of Animal Morphology And Function Graduate School Of Bioagricultural Sciences Nagoya Univ.

• Takagi Nobuo

  Graduate School of Environmental Earth Science, Hokkaido University

• Atsumi Tadao

  Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN)

• Kawakura Kazuhiko

  National Institute of Animal Industry

• Kusakabe Moriaki

  Tsukuba Life Science Center, The Institute of Physical and Chemical Research (RIKEN)

• Atsumi Tadao

  Laboratory Of Molecular Cell Science Tsukuba Life Science Center The Institute Of Physical And Chemi

• Takagi Nobuo

  Division Of Bioscience Graduate School Of Environmental Earth Science Hokkaido University

• Kusakabe M

  Aloka Co. Ltd. Ibaraki

• Takagi Nobuo

  Graduate School Of Environmental Earth Science Hokkaido University

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