Identification Procedure for Pasteurella Pneumotropica in Microbiologic Monitoring of Laboratory Animals

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概要

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• Discrepancies have been recognized in the identification of Pasteurella pneumotropica between testing laboratories. To determine the causes of the differences and to propose a reliable identification procedure for P. pneumotropica, a working group was organized and 69 isolates identified or suspected as P. pneumotropica were collected from 8 laboratories in Japan. These isolates were examined by colony morphology, Gram-staining, the slide agglutination test using two antisera (ATCC35149 and MaR), two commercially available biochemical test kits (ID test, API20NE) and two primer sets of PCR tests (Wang PCR, CIEA PCR). The 69 isolates and two reference strains were divided into 10 groups by test results. No single procedure for P. pneumotropica identification was found. Among tested isolates, large differences were not observed by colony morphology and Gram-straining except for colony colors that depended on their biotypes. Sixty-eight out of 69 isolates were positive by the slide agglutination test using two antisera except for one isolate that tested with one antiserum. The ID test identified 61 out of 69 isolates as P. pneumotropica and there was no large difference from the results of CIEA PCR. From these results, we recommend the combination of colony observation, Gram-straining, the slide agglutination tests with two antisera and biochemical test using the ID test for practical and reliable identification of this organism.

• 社団法人　日本実験動物学会の論文
• 2005-04-01

著者

• TAKAKURA Akira

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• Aiba Takeshi

  Pharmaceuticals and Medical Devices Agency

• Itoh T

  Iclas Monitoring Center Central Institute For Experimental Animals

• Takakura A

  Iclas Monitoring Center Central Institute For Experimental Animals

• ITOH Kikuji

  Laboratory of Veterinary Public Health, Graduate School of Agriculture and Life Sciences, The Univer

• 高倉 彰

  財団法人実験動物中央研究所

• Takakura Akira

  Iclas Monitoring Center Central Institute For Experimental Animals

• Takakura Akira

  実験動物中央研究所 Iclasモニタリングセンター

• Narita Teruo

  Medicinal Safety Research Laboratory Sankyo Co. Ltd.

• ITOH Toshio

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• Aiba Takeshi

  Pharmacodynamics Research Laboratories Sankyo Co. Ltd.

• HAYASHIMOTO Nobuhito

  ICLAS Monitoring Center, Central Institute for Experimental Animals

• KATO Megumi

  Charles River Japan, Inc.

• KAWAMOTO Eiichi

  Animal Research Center, Tokyo Medical University

• KIYOKAWA Sumito

  CLEA JAPAN, Inc.

• MORICHIKA Yoko

  Japan SLC, Inc.

• MURAGUCHI Takehiko

  Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University

• OKAJIMA Yasuo

  Environmental Research Center, Mercian Cleantec Corporation

• KATO Megumi

  National Institute for Physiological Sciences

• Itoh Toshio

  Iclas Monitoring Center Central Institute For Experimental Animals

• Okajima Yasuo

  Environmental Research Center Mercian Cleantec Corporation

• Kiyokawa Sumito

  Clea Japan Inc.

• Morichika Yoko

  Japan Slc Inc.

• Itoh Kikuji

  Laboratory Of Veterinary Public Health

• Kato M

  National Institute For Physiological Sciences

• Kato Megumi

  Charles River Japan Inc.

• Muraguchi Takehiko

  Institute Of Laboratory Animals Graduate School Of Medicine Kyoto University

• Kawamoto Eiichi

  Animal Research Center Tokyo Medical University

• Takashima Ikuo

  Hokkaido Univ. Hokkaido Jpn

• Itoh Kikuji

  Laboratory Of Veterinary Public Health Graduate School Of Agricultural And Life Sciences University

• Hayashimoto Nobuhito

  Iclas Monitoring Center Central Institute For Experimental Animals

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