Genotyping of Mouse Hepatitis Virus Strains by Restriction Endonuclease Analysis of Amplified Nucleocapsid Protein Genes
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概要
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Restriction endonuclease analysis of amplified nucleocapsid protein genes from mouse hepatitis virus (MHV) was used to differentiate 12 strains isolated from mouse liver or transplantable tumors from five facilities, and the restriction patterns of the isolates were compared with those of five well-defined MHV strains, A59, JHM, 2, S and Nu-67. The patterns of 10 isolates from three facilities were the same as that of Nu-67. The remaining two isolates revealed different patterns from the five reference strains. This study showed that reverse transcription and the polymerase chain reaction assay based restriction analysis are feasible for the detection and genotyping of MHV, and the Nu-67 related strain was the most prevalent type found in the clinical samples.
- 社団法人 日本実験動物学会の論文
著者
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Itoh T
Iclas Monitoring Center Central Institute For Experimental Animals
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Kunita Satoshi
筑波大学生命科学動物資源センター
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Kunita Satoshi
Laboratory Animal Resource Center University Of Tsukuba
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Kunita Satoshi
実験動物中央研究所
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Kunita Satoshi
Institute Of Experimental Animal Research School Of Science Kitasato University
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ITOH Toshio
Central Institute for Experimental Animals
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Goto Kazuo
Iclas Monitoring Center Central Institute For Experimental Animals
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GOTO Kazuo
Central Institute for Experimental Animals
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NOZU Ryoko
Central Institute for Experimental Animals
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TERADA Eiji
Institute of Experimental Animal Research, School of Science, Kitasato University
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GOTOH Kazuo
Central Institute for Experimental Animals
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Goto Kazuo
Department Of Genetics International Council For Laboratory Animal Science Monitoring Center Central
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Nozu Ryoko
Central Institute For Experimntal
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Terada Eiji
Institute Of Experimental Animal Research School Of Science Kitasato University
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Takashima Ikuo
Hokkaido Univ. Hokkaido Jpn
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